Severe acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated protein

	Severe acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated protein
	Xu, K; Zheng, BJ; Zeng, R; Lu, W; Lin, YP; Xue, L; Li, L; Yang, LL; Xu, C; Dai, J
刊名	VIROLOGY
	2009
卷号	388 期号:2 页码:279-285
关键词	SARS-CoV ORF9b Virion-associated protein
通讯作者	Wu, JR (reprint author), Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,wujr@sibs.ac.cn ; bsun@sibs.ac.cn
英文摘要	Eight accessory proteins have been identified in severe acute respiratory syndrome-associated coronavirus (SARS-CoV). They are believed to play roles in the viral life cycle and may contribute to the pathogenesis and virulence. ORF9b as one of these accessory proteins is located in subgenomic mRNA9 and encodes a 98 amino acid protein. However, whether 9b protein is a Structural component of SARS-CoV particles remains unknown. In this study, we demonstrate that 9b protein is translated from bicistronic mRNA9 via leaky ribosome scanning and it is incorporated into both virus-like particles (VLPs) and purified SARS-CoV virions. Further analysis shows that sufficient incorporation of 9b protein into VLPs is dependent upon the co-expression of E and M proteins, but not upon the presence of either S or N protein. Our data indicate that 9b protein of SARS-CoV is another virion-associated accessory protein. This finding will lead to a better understanding of the properties of the SARS-CoV 9b protein. (c) 2009 Elsevier Inc. All rights reserved.
学科主题	Virology
类目[WOS]	Virology
关键词[WOS]	SARS-ASSOCIATED CORONAVIRUS ; VIRAL STRUCTURAL PROTEIN ; VIRUS-INFECTED CELLS ; AUG INITIATOR CODON ; OPEN READING FRAME ; 3A PROTEIN ; EXPRESSING CELLS ; 7A PROTEIN ; TRANSLATION ; PARTICLES
收录类别	SCI
语种	英语
WOS记录号	WOS:000266621400006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1143]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xu, K,Zheng, BJ,Zeng, R,et al. Severe acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated protein[J]. VIROLOGY,2009,388(2):279-285.
APA	Xu, K.,Zheng, BJ.,Zeng, R.,Lu, W.,Lin, YP.,...&Sun, B.(2009).Severe acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated protein.VIROLOGY,388(2),279-285.
MLA	Xu, K,et al."Severe acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated protein".VIROLOGY 388.2(2009):279-285.