Safflower extracts functionally regulate monoamine transporters

	Safflower extracts functionally regulate monoamine transporters
	Zhao, G; Zheng, XW; Gai, Y; Chu, WJ; Qin, GW; Guo, LH
刊名	JOURNAL OF ETHNOPHARMACOLOGY
	2009
卷号	124 期号:1 页码:116-124
关键词	Safflower Dopamine Norepinephrine Serotonin Reuptake modulator Transporter
通讯作者	Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,lhguo@sibs.ac.cn
英文摘要	Safflower (HH), the dry flower of Carthamus tinctorius L, has long been used to empirically treat neuropsychological disorders such as stroke and major depression in traditional Chinese medicine, and recently been proven effective for regulating levels of dopamine and serotonin in new-born rat brain. The present study assessed whether HH would be bioactive for functionally regulating monoamine transporters using in vitro drug-screening cell lines. Our current results showed that all solvent-extracted HH fractions, in different degrees, markedly increased both dopamine uptake by Chinese hamster ovary (CHO) cells stably expressing dopamine transporter (DAT) and norepinephrine uptake by CHO cells expressing norepinephrine transporter (NET), and also showed that chloroform (HC), ethyl acetate (HE), and n-butyl alcohol extract strikingly depressed serotonin uptake by CHO cells expressing serotonin transporter (SERT); wherein, the potencies of ethanol extract, HC, HE, and aqueous extract to up-regulate dopamine/norepinephrine uptake and potency of HE to inhibit serotonin uptake were relatively stronger. Further investigation revealed that the enhancement of dopamine/norepinephrine uptake by HC and HE was dependent of DAT/NET activity, and the HE-induced inhibition of serotonin uptake was typical of competition. Thus, HH extracts are novel monoamine transporter modulators functioning as DAT/NET activators and/or SERT inhibitors, and would likely improve neuropsychological disorders through regulating monoamine-transporter activity. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
学科主题	Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine
类目[WOS]	Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
关键词[WOS]	DOPAMINE TRANSPORTER ; CARTHAMUS-TINCTORIUS ; HYDROXYSAFFLOR-YELLOW ; SEROTONIN DERIVATIVES ; LINOLENIC ACID ; RAT-BRAIN ; IN-VITRO ; MICE ; DYSFUNCTION ; BEHAVIOR
收录类别	SCI
语种	英语
WOS记录号	WOS:000268201200014
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1118]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhao, G,Zheng, XW,Gai, Y,et al. Safflower extracts functionally regulate monoamine transporters[J]. JOURNAL OF ETHNOPHARMACOLOGY,2009,124(1):116-124.
APA	Zhao, G,Zheng, XW,Gai, Y,Chu, WJ,Qin, GW,&Guo, LH.(2009).Safflower extracts functionally regulate monoamine transporters.JOURNAL OF ETHNOPHARMACOLOGY,124(1),116-124.
MLA	Zhao, G,et al."Safflower extracts functionally regulate monoamine transporters".JOURNAL OF ETHNOPHARMACOLOGY 124.1(2009):116-124.