Develop reusable and combinable designs for transcriptional logic gates

	Develop reusable and combinable designs for transcriptional logic gates
	Zhan, JA; Ding, B; Ma, R; Ma, XY; Su, XF; Zhao, Y; Liu, ZQ; Wu, JR; Liu, HY
刊名	MOLECULAR SYSTEMS BIOLOGY
	2010
卷号	6 期号:1 页码:388-388
关键词	combinability mathematical model reusable designs transcriptional logic gates
通讯作者	Liu, HY (reprint author), Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China.,hyliu@ustc.edu.cn
英文摘要	One limit on developing complex synthetic gene circuits is the lack of basic components such as transcriptional logic gates that can process combinatorial inputs. Here, we propose a strategy to construct such components based on reusable designs and convergent reengineering of well-studied natural systems. We demonstrated the strategy using variants of the transcription factor (TF) LacI and operator Olac that form specifically interacting pairs. Guided by a mathematical model derived from existing quantitative knowledge, rational designs of transcriptional NAND, NOR and NOT gates have been realized. The NAND gates have been designed based on direct protein-protein interactions in coupling with DNA looping. We demonstrated that the designs are reusable: a multiplex of logic devices can be readily created using the same designs but different combinations of sequence variants. The designed logic gates are combinable to form compound circuits: a demonstration logic circuit containing all three types of designed logic gates has been synthesized, and the circuit truthfully reproduces the pre-designed input-output logic relations. Molecular Systems Biology 6: 388; published online 13 July 2010; doi:10.1038/msb.2010.42 Subject Categories: synthetic biology
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	LAC REPRESSOR ; DNA-BINDING ; CRYSTAL-STRUCTURE ; SPECIFICITY ; OPERATOR ; RECOGNITION ; MUTANTS ; CONSTRUCTION ; DIMERIZATION ; OSCILLATOR
收录类别	SCI
语种	英语
WOS记录号	WOS:000284524200009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1106]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhan, JA,Ding, B,Ma, R,et al. Develop reusable and combinable designs for transcriptional logic gates[J]. MOLECULAR SYSTEMS BIOLOGY,2010,6(1):388-388.
APA	Zhan, JA.,Ding, B.,Ma, R.,Ma, XY.,Su, XF.,...&Liu, HY.(2010).Develop reusable and combinable designs for transcriptional logic gates.MOLECULAR SYSTEMS BIOLOGY,6(1),388-388.
MLA	Zhan, JA,et al."Develop reusable and combinable designs for transcriptional logic gates".MOLECULAR SYSTEMS BIOLOGY 6.1(2010):388-388.