Tripartite-Motif Protein 30 Negatively Regulates NLRP3 Inflammasome Activation by Modulating Reactive Oxygen Species Production

	Tripartite-Motif Protein 30 Negatively Regulates NLRP3 Inflammasome Activation by Modulating Reactive Oxygen Species Production
	Hu, Y; Mao, KR; Zeng, Y; Chen, SZ; Tao, ZY; Yang, C; Sun, SH; Wu, XD; Meng, GX; Sun, B
刊名	JOURNAL OF IMMUNOLOGY
	2010
卷号	185 期号:12 页码:7699-7705
通讯作者	Sun, B (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,bsun@sibs.ac.cn
英文摘要	The NLR family, pyrin domain-containing 3 (NLRP3) inflammasome is critical for caspase-1 activation and the proteolytic processing of pro-IL-1 beta. However, the mechanism that regulates NLRP3 inflammasome activation remains unclear. In this paper, we demonstrate that tripartite-motif protein 30 (TRIM30) negatively regulates NLRP3 inflammasome activation. After stimulation with ATP, an agonist of the NLRP3 inflammasome, knockdown of TRIM30 enhanced caspase-1 activation and increased production of IL-1 beta in both J774 cells and bone marrow-derived macrophages. Similarly with ATP, knockdown of TRIM30 increased caspase-1 activation and IL-1 beta production triggered by other NLRP3 inflammasome agonists, including nigericin, monosodium urate, and silica. Production of reactive oxygen species was increased in TRIM30 knockdown cells, and its increase was required for enhanced NLRP3 inflammasome activation, because antioxidant treatment blocked excess IL-1 beta production. Conversely, overexpression of TRIM30 attenuated reactive oxygen species production and NLRP3 inflammasome activation. Finally, in a crystal-induced NLRP3 inflammasome-dependent peritonitis model, monosodium urate-induced neutrophil flux and IL-1 beta production was reduced significantly in TRIM30 transgenic mice as compared with that in their nontransgenic littermates. Taken together, our results indicate that TRIM30 is a negative regulator of NLRP3 inflammasome activation and provide insights into the role of TRIM30 in maintaining inflammatory responses. The Journal of Immunology, 2010, 185: 7699-7705.
学科主题	Immunology
类目[WOS]	Immunology
关键词[WOS]	TOLL-LIKE RECEPTOR ; NALP3 INFLAMMASOME ; IL-1-BETA PRODUCTION ; PATTERN-RECOGNITION ; CRYSTALS ; ADAPTERS ; SILICA ; CASPASE-1 ; PLATFORM ; FAMILY
收录类别	SCI
语种	英语
WOS记录号	WOS:000284878700069
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1074]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hu, Y,Mao, KR,Zeng, Y,et al. Tripartite-Motif Protein 30 Negatively Regulates NLRP3 Inflammasome Activation by Modulating Reactive Oxygen Species Production[J]. JOURNAL OF IMMUNOLOGY,2010,185(12):7699-7705.
APA	Hu, Y.,Mao, KR.,Zeng, Y.,Chen, SZ.,Tao, ZY.,...&Sun, B.(2010).Tripartite-Motif Protein 30 Negatively Regulates NLRP3 Inflammasome Activation by Modulating Reactive Oxygen Species Production.JOURNAL OF IMMUNOLOGY,185(12),7699-7705.
MLA	Hu, Y,et al."Tripartite-Motif Protein 30 Negatively Regulates NLRP3 Inflammasome Activation by Modulating Reactive Oxygen Species Production".JOURNAL OF IMMUNOLOGY 185.12(2010):7699-7705.