A new subfamily of conotoxins belonging to the A-superfamily

	A new subfamily of conotoxins belonging to the A-superfamily
	Peng, C; Ye, MY; Wang, YF; Shao, XX; Yuan, DD; Liu, J; Hawrot, E; Wang, CG; Chi, CW
刊名	PEPTIDES
	2010
卷号	31 期号:11 页码:2009-2016
关键词	Conotoxins A superfamily Cysteine framework 14 Nicotinic acetylcholine receptors
通讯作者	Wang, CG (reprint author), Tongji Univ, Coll Life Sci & Technol, Inst Prot Res, 1239 Siping Rd, Shanghai 200092, Peoples R China.,
英文摘要	Two novel conotoxins from vermivorous cone snails Conus pulicarius and Conus tessulatus designated as Pu14 1 and ts14a were identified by cDNA cloning and peptide purification respectively The signal sequence of Pu14 1 is identical to that of alpha-conotoxins while its predicted mature peptide pu14a shares high sequence similarity with ts14a with only one residue different in their first intercysteine loop which contains 10 residues and is rich in proline Both pu14a and ts14a contain four separate cysteines in framework 14 (C-C-C-C) Peptide pu14a was chemically synthesized air oxidized and the connectivity of its two disulfide bonds was determined to be C1-C3 C2-C4 which is the same as found in alpha-conotoxins The synthetic pu14a induced a sleeping symptom in mice and was toxic to freshwater goldfish upon intramuscular injection Using the Xenopus oocyte heterologous expression system 1 mu M of pu14a demonstrated to inhibit the rat neuronal alpha 3 beta 2-containing as well as the mouse neuromuscular alpha 1 beta 1 gamma delta subtypes of nicotinic acetylcholine receptors and then rapidly dissociated from the receptors However this toxin had no inhibitory effect on potassium channels in mouse superior cervical ganglion neurons According to the identical signal sequence to alpha-conotoxins the unique cysteine framework and molecular target of pu14a we propose that pu14a and ts14a may represent a novel subfamily in the A-superfamily designated as alpha 1-conotoxins (C) 2010 Elsevier Inc All rights reserved
学科主题	Biochemistry & Molecular Biology; Pharmacology & Pharmacy
类目[WOS]	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]	NICOTINIC ACETYLCHOLINE-RECEPTOR ; UNIQUE CYSTEINE PATTERN ; CHEMICAL-SYNTHESIS ; SCORPION TOXIN ; NMR STRUCTURE ; K+ CHANNELS ; IDENTIFICATION ; ANTAGONIST ; ASSIGNMENT ; INHIBITOR
收录类别	SCI
语种	英语
WOS记录号	WOS:000284501800008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1071]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Peng, C,Ye, MY,Wang, YF,et al. A new subfamily of conotoxins belonging to the A-superfamily[J]. PEPTIDES,2010,31(11):2009-2016.
APA	Peng, C.,Ye, MY.,Wang, YF.,Shao, XX.,Yuan, DD.,...&Chi, CW.(2010).A new subfamily of conotoxins belonging to the A-superfamily.PEPTIDES,31(11),2009-2016.
MLA	Peng, C,et al."A new subfamily of conotoxins belonging to the A-superfamily".PEPTIDES 31.11(2010):2009-2016.