Dishevelled interacts with p65 and acts as a repressor of NF-kappa B-mediated transcription

	Dishevelled interacts with p65 and acts as a repressor of NF-kappa B-mediated transcription
	Deng, N; Ye, YE; Wang, W; Li, L
刊名	CELL RESEARCH
	2010
卷号	20 期号:10 页码:1117-1127
关键词	Dishevelled (Dvl) NF-kappa B p65 transcriptional suppressor Wnt signaling
通讯作者	Li, L (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,lli@sibs.ac.cn
英文摘要	Dishevelled (Dvl) is a highly conserved protein family that plays an important role in mediating Wnt signaling from membrane to cytoplasm. Recently we reported that Dvl also functions in the nucleus by stabilizing the beta-catenin/TCFs transcriptional complex. Here we describe that Dvl may function as a repressor of NF-kappa B. Our data show that Dvl directly binds to p65 and their interaction occurs in the nucleus. Dvl expression inhibits p65-mediated or TNF-alpha-stimulated activation of the NF-kappa B dependent reporter. This action of Dvl, however, is not dependent on Wnt or its downstream effector beta-catenin. Chromatin immunoprecipitation assay shows that recruitment of p65 to the promoters of NF-kappa B target genes is significantly enhanced when expression of Dvl is knocked down. Consistently, the expression level of a subset of NF-kappa B target genes is also increased after knock-down of Dvl. Moreover, our data suggest that Dvl may relieve the anti-apoptotic effect of NF-kappa B, thus play a role in promoting apoptosis. Therefore, this work demonstrates a novel function of Dvl in modulating NF-kappa B-regulated gene transcription.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	BETA-CATENIN ; SIGNALING PATHWAYS ; INDUCED APOPTOSIS ; CELL POLARITY ; C-JUN ; PROTEINS ; CANCER ; A20 ; ACTIVATION ; MECHANISMS
收录类别	SCI
语种	英语
WOS记录号	WOS:000283397100007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1057]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Deng, N,Ye, YE,Wang, W,et al. Dishevelled interacts with p65 and acts as a repressor of NF-kappa B-mediated transcription[J]. CELL RESEARCH,2010,20(10):1117-1127.
APA	Deng, N,Ye, YE,Wang, W,&Li, L.(2010).Dishevelled interacts with p65 and acts as a repressor of NF-kappa B-mediated transcription.CELL RESEARCH,20(10),1117-1127.
MLA	Deng, N,et al."Dishevelled interacts with p65 and acts as a repressor of NF-kappa B-mediated transcription".CELL RESEARCH 20.10(2010):1117-1127.