Deletion of EFG1 promotes Candida albicans opaque formation responding to pH via Rim101

	Deletion of EFG1 promotes Candida albicans opaque formation responding to pH via Rim101
	Nie, XY; Liu, XY; Wang, HF; Chen, JY
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2010
卷号	42 期号:10 页码:735-744
关键词	pH regulation white-opaque switching Efg1 Rim101 Candida albicans
通讯作者	Chen, JY (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,jychen@sibs.ac.cn
英文摘要	Phenotypic switching in Candida albicans spontaneously generates different cellular morphologies. The reversible switching between white and opaque phenotypes is regulated by multiple regulators including Efg1 and Wor1. In mating-type-like locus (MTL) homozygous cells, the Efg1 functions as a repressor, whereas the Wor1 acts as an activator in white-opaque switching. We presented evidence that switching between white and opaque in efg1/efg1 mutant is regulated by ambient pH. In pH 6.8 media, the efg1/efg1 mutant cells exhibited opaque form, but shifted to white form in pH 4.5 media. The pH-dependent morphological switching is not blocked by further deletion of WOR1 in the efg1/efg1 mutant. Correlated with the phenotype, the opaque-phase-specific gene OP4 was induced in efg1/efg1 mutant cells when cultured in pH 6.8 media, and was repressed in pH 4.5 media. Consistently, the MTLa efg1/efg1 mutant cells could mate efficiently with MTL alpha cells in pH 6.8 media, but poorly in pH 4.5 media. Ectopic expression of the Rim101-405 allele in the efg1/efg1 mutant helped to bypass the pH restriction on white-opaque switching and show opaque form in both neutral and acidic media. We proposed that relief of the Efg1 repression enables C. albicans to undergo white-opaque switching in pH-dependent regulation mediated by Rim101-signaling pathway.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	TRANSCRIPTION FACTOR ; GENE-EXPRESSION ; MORPHOGENESIS ; YEAST ; REGULATOR ; PROTEINS ; IDENTIFICATION ; FILAMENTATION ; VIRULENCE ; MUTANTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000282166500008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1055]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Nie, XY,Liu, XY,Wang, HF,et al. Deletion of EFG1 promotes Candida albicans opaque formation responding to pH via Rim101[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2010,42(10):735-744.
APA	Nie, XY,Liu, XY,Wang, HF,&Chen, JY.(2010).Deletion of EFG1 promotes Candida albicans opaque formation responding to pH via Rim101.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,42(10),735-744.
MLA	Nie, XY,et al."Deletion of EFG1 promotes Candida albicans opaque formation responding to pH via Rim101".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 42.10(2010):735-744.