Structural Transformation of the Tandem Ubiquitin-Interacting Motifs in Ataxin-3 and Their Cooperative Interactions with Ubiquitin Chains

	Structural Transformation of the Tandem Ubiquitin-Interacting Motifs in Ataxin-3 and Their Cooperative Interactions with Ubiquitin Chains
	Song, AX; Zhou, CJ; Peng, Y; Gao, XC; Zhou, ZR; Fu, QS; Hong, J; Lin, DH; Hu, HY
刊名	PLOS ONE
	2010
卷号	5 期号:10 页码:e13202-e13202
通讯作者	Song, AX (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai, Peoples R China.,dhlin@mail.shcnc.ac.cn ; hyhu@sibs.ac.cn
英文摘要	The ubiquitin-interacting motif (UIM) is a short peptide with dual function of binding ubiquitin (Ub) and promoting ubiquitination. We elucidated the structures and dynamics of the tandem UIMs of ataxin-3 (AT3-UIM12) both in free and Ub-bound forms. The solution structure of free AT3-UIM12 consists of two alpha-helices and a flexible linker, whereas that of the Ub-bound form is much more compact with hydrophobic contacts between the two helices. NMR dynamics indicates that the flexible linker becomes rigid when AT3-UIM12 binds with Ub. Isothermal titration calorimetry and NMR titration demonstrate that AT3-UIM12 binds diUb with two distinct affinities, and the linker plays a critical role in association of the two helices in diUb binding. These results provide an implication that the tandem UIM12 interacts with Ub or diUb in a cooperative manner through an allosteric effect and dynamics change of the linker region, which might be related to its recognitions with various Ub chains and ubiquitinated substrates.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	POLYGLUTAMINE DISEASE PROTEIN ; DEUBIQUITINATING ENZYME ATAXIN-3 ; MACHADO-JOSEPH-DISEASE ; NMR SOLUTION STRUCTURE ; BINDING DOMAINS ; CRYSTAL-STRUCTURE ; CHEMICAL-SHIFT ; RECOGNITION ; SYSTEM ; PROTEASOME
收录类别	SCI
语种	英语
WOS记录号	WOS:000282633700009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1052]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Song, AX,Zhou, CJ,Peng, Y,et al. Structural Transformation of the Tandem Ubiquitin-Interacting Motifs in Ataxin-3 and Their Cooperative Interactions with Ubiquitin Chains[J]. PLOS ONE,2010,5(10):e13202-e13202.
APA	Song, AX.,Zhou, CJ.,Peng, Y.,Gao, XC.,Zhou, ZR.,...&Hu, HY.(2010).Structural Transformation of the Tandem Ubiquitin-Interacting Motifs in Ataxin-3 and Their Cooperative Interactions with Ubiquitin Chains.PLOS ONE,5(10),e13202-e13202.
MLA	Song, AX,et al."Structural Transformation of the Tandem Ubiquitin-Interacting Motifs in Ataxin-3 and Their Cooperative Interactions with Ubiquitin Chains".PLOS ONE 5.10(2010):e13202-e13202.