High-efficiency somatic reprogramming induced by intact MII oocytes

	High-efficiency somatic reprogramming induced by intact MII oocytes
	Yang, H; Shi, LY; Zhang, SH; Lin, JW; Jiang, J; Li, JS
刊名	CELL RESEARCH
	2010
卷号	20 期号:9 页码:1034-1042
关键词	somatic reprogramming nuclear transfer intact oocytes embryonic stem cell tetraploid
通讯作者	Li, JS (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Mol Cell Biol Lab, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,jsli@sibs.ac.cn
英文摘要	Somatic nuclei can be reprogrammed into a pluripotent state by nuclear transfer, cell fusion and expression of transcription factors. However, these reprogramming processes are very inefficient, which has greatly hindered efforts to elucidate the underlying molecular mechanisms. Here, we report a new reprogramming strategy that combines the advantages of all three reprogramming methodologies into one process. We injected nuclei from cumulus cells into intact MII oocytes. Following activation, 80% of the reconstructed embryos developed to the blastocyst stage, and tetraploid (4N) embryonic stem (ES) cell lines were generated at a rate of 30% per reconstructed oocyte. We also generated triploid (3N) ES cells after injection of somatic nuclei into activated oocytes. 4N and 3N ES cells expressed pluripotent markers and differentiated into cell types of three embryonic germ layers in vivo. Moreover, all ES cells generated histocompatible, differentiated cells after being engrafted in immunocompetent B6D2F1 mice, showing that ES cells derived from this reprogramming strategy might serve as a source of genetically tailored tissues for transplantation. Thus, we have established a simple and highly efficient reprogramming procedure that provides a system for investigating the molecular mechanisms involved in somatic reprogramming.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	EMBRYONIC STEM-CELLS ; NUCLEAR TRANSFER ; HUMAN FIBROBLASTS ; MOUSE ZYGOTES ; PLURIPOTENCY ; MICE ; CLONING ; ESTABLISHMENT ; FUSION ; CLONES
收录类别	SCI
语种	英语
WOS记录号	WOS:000281483800009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1047]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yang, H,Shi, LY,Zhang, SH,et al. High-efficiency somatic reprogramming induced by intact MII oocytes[J]. CELL RESEARCH,2010,20(9):1034-1042.
APA	Yang, H,Shi, LY,Zhang, SH,Lin, JW,Jiang, J,&Li, JS.(2010).High-efficiency somatic reprogramming induced by intact MII oocytes.CELL RESEARCH,20(9),1034-1042.
MLA	Yang, H,et al."High-efficiency somatic reprogramming induced by intact MII oocytes".CELL RESEARCH 20.9(2010):1034-1042.