Label-free detection of hybridization of oligonucleotides by oblique-incidence reflectivity difference method

	Label-free detection of hybridization of oligonucleotides by oblique-incidence reflectivity difference method
	Yuan, K; Wang, X; Lu, H; Wen, JA; Lu, HB; Zhou, YL; Jin, KJ; Yang, GZ; Li, W; Ruan, KC
刊名	SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY
	2010
卷号	53 期号:8 页码:1434-1437
关键词	oblique-incidence reflectivity difference microarray detection label-free detection oligonucleotides microarray
通讯作者	Lu, HB (reprint author), Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China.,hblu@aphy.iphy.ac.cn ; kcruan@sibs.ac.cn
英文摘要	The microarrays of 20-base oligonucleotide with different concentrations are detected before and after hybridization by the oblique-incidence reflectivity difference (OI-RD) method. The experimental results prove that OI-RD is a label-free method which can not only distinguish the concentration difference of oligonucleotides before and after the hybridization but also detect the hybridization of short oligonucleotides. At present the OI-RD method can detect 0.39 mu mol/L 20-base oligonucleotide or less. These results suggest that the OI-RD method is a promising and potential technique for label-free detection of biological microarrays.
学科主题	Physics
类目[WOS]	Physics, Multidisciplinary
关键词[WOS]	DNA MICROARRAY
收录类别	SCI
语种	英语
WOS记录号	WOS:000280477200008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1039]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yuan, K,Wang, X,Lu, H,et al. Label-free detection of hybridization of oligonucleotides by oblique-incidence reflectivity difference method[J]. SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,2010,53(8):1434-1437.
APA	Yuan, K.,Wang, X.,Lu, H.,Wen, JA.,Lu, HB.,...&Ruan, KC.(2010).Label-free detection of hybridization of oligonucleotides by oblique-incidence reflectivity difference method.SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,53(8),1434-1437.
MLA	Yuan, K,et al."Label-free detection of hybridization of oligonucleotides by oblique-incidence reflectivity difference method".SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY 53.8(2010):1434-1437.