A Study on The Mechanism of The Inhibition of Bel-7404 Hepatocarcinoma Cell Growth by MG132

	A Study on The Mechanism of The Inhibition of Bel-7404 Hepatocarcinoma Cell Growth by MG132
	Zhang, J; Li, W; Zhang, KJ; Liu, XJ; Kong, YP; Niu, N; Jiang, H; Zhou, XM
刊名	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
	2010
卷号	37 期号:6 页码:627-634
关键词	proteasome inhibitor Bel-7404 cells apoptosis autophagy ER stress
通讯作者	Zhou, XM (reprint author), Zhejiang Sci Tech Univ, Coll Life Sci, Xin Yuan Inst Med & Biotechnol, Hangzhou 310018, Peoples R China.,zhouxiumei824@163.com
英文摘要	MG132(Z-Leu-leu-leu-CHO) is an inhibitor of proteasome, and it can reversibly inhibit the activation of proteasome, thereby inhibiting the degradation of protein which involved in ubiquitin-proteasome pathway (UPP), and inducing apoptosis at last. Study demonstrated that MG132 was capable of inhibition the proliferation of Bel-7404 hepatocarcinoma cell. After treated with different-concentration of MG132 at different-time, the change of morphological change and endoplasmic reticulum stress, formation of autophagic vacuoles and apoptotic bodies, cells viability, cell apoptosis, the protein expression of both apoptosis and autophagy signaling pathway related genes formation of in Bel-7404 cells were assessed by fluorescence microscope, Hoechst33342 staining, MTT assay, Annexin V /PI flow cytometry, Western blotting and transmission electron microscopy analysis. The results suggested that MG132 can inhibit Bel-7404 cells growth remarkably. It was able to activate Caspase-12 through endoplasmic reticulum stress pathway, can also influence the level of Bcl-2/Bax, and consequently induced releasing of cytochrome c through mitochondria] pathway. Both of the two different signaling pathways can activate Caspase-3 and PARP. Furthermore, MG132 increased the expression of Beclin 1 and LC3B. Autophagic vacuoles were also detected by transmission electron microscopy analysis. It was confirmed that MG132 inhibited the growth of Bel-7404 cells not only via apoptosis pathway, but also related with autophagy pathway.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	LUNG-CANCER CELLS ; PROTEASOME INHIBITOR ; SENSITIZES CELLS ; APOPTOSIS ; UBIQUITIN ; AUTOPHAGY ; BORTEZOMIB ; MEMBRANES ; PROTEINS ; CLEAVAGE
收录类别	SCI
语种	中文
WOS记录号	WOS:000279312300008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1019]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, J,Li, W,Zhang, KJ,et al. A Study on The Mechanism of The Inhibition of Bel-7404 Hepatocarcinoma Cell Growth by MG132[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2010,37(6):627-634.
APA	Zhang, J.,Li, W.,Zhang, KJ.,Liu, XJ.,Kong, YP.,...&Zhou, XM.(2010).A Study on The Mechanism of The Inhibition of Bel-7404 Hepatocarcinoma Cell Growth by MG132.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,37(6),627-634.
MLA	Zhang, J,et al."A Study on The Mechanism of The Inhibition of Bel-7404 Hepatocarcinoma Cell Growth by MG132".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 37.6(2010):627-634.