Hepatoblast-Like Progenitor Cells Derived From Embryonic Stem Cells Can Repopulate Livers of Mice

	Hepatoblast-Like Progenitor Cells Derived From Embryonic Stem Cells Can Repopulate Livers of Mice
	Li, FM; Liu, PY; Liu, CC; Xiang, D; Deng, L; Li, WL; Wangensteen, K; Song, JG; Ma, Y; Hui, LJ
刊名	GASTROENTEROLOGY
	2010
卷号	139 期号:6 页码:2158-U495
关键词	Hepatic Differentiation Bipotency Cell Transplantation Liver Injury
通讯作者	Wang, X (reprint author), Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,zyhe@smmu.edu.cn ; wangx@sibs.ac.cn
英文摘要	BACKGROUND & AIMS: Hepatocyte-like cells can be derived from pluripotent stem cells such as embryonic stem (ES) cells, but ES cell-derived hepatic cells with extensive capacity to repopulate liver have not been identified. We aimed to identify and purify ES cell-derived hepatoblast-like progenitor cells and to explore their capacity for liver repopulation in mice after in vitro expansion. METHODS: Unmanipulated mouse ES cells were cultured under defined conditions and allowed to undergo stepwise hepatic differentiation. The derived hepatic cells were examined by morphologic, fluorescence-activated cell sorting, gene expression, and clonal expansion analyses. The capacities of ES cell-derived hepatic progenitor cells to repopulate liver were investigated in mice that were deficient in fumarylacetoacetate hydrolase (Fah) (a model of liver injury). RESULTS: Mouse ES cells were induced to differentiate into a population that contained hepatic progenitor cells; this population included cells that expressed epithelial cell adhesion molecule (EpCAM) but did not express c-Kit. Clonal hepatic progenitors that arose from single c-Kit(-)EpCAM(+) cells could undergo long-term expansion and maintain hepatoblast-like characteristics. Enriched c-Kit(-)EpCAM(+) cells and clonally expanded hepatic progenitor cells repopulated the livers of Fah-deficient mice without inducing tumorigenesis. CONCLUSIONS: ES cell-derived c-Kit(-)EpCAM(+) cells contain a population of hepatoblast-like progenitor cells that can repopulate livers of mice.
学科主题	Gastroenterology & Hepatology
类目[WOS]	Gastroenterology & Hepatology
关键词[WOS]	MOUSE-LIVER ; IN-VIVO ; EFFICIENT DIFFERENTIATION ; DEFINITIVE ENDODERM ; HEPATIC ENDODERM ; HEPATOCYTES ; GENERATION ; ENRICHMENT ; TRANSPLANTATION ; REGENERATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000284843600053
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1013]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, FM,Liu, PY,Liu, CC,et al. Hepatoblast-Like Progenitor Cells Derived From Embryonic Stem Cells Can Repopulate Livers of Mice[J]. GASTROENTEROLOGY,2010,139(6):2158-U495.
APA	Li, FM.,Liu, PY.,Liu, CC.,Xiang, D.,Deng, L.,...&Wang, X.(2010).Hepatoblast-Like Progenitor Cells Derived From Embryonic Stem Cells Can Repopulate Livers of Mice.GASTROENTEROLOGY,139(6),2158-U495.
MLA	Li, FM,et al."Hepatoblast-Like Progenitor Cells Derived From Embryonic Stem Cells Can Repopulate Livers of Mice".GASTROENTEROLOGY 139.6(2010):2158-U495.