tRNA-dependent Pre-transfer Editing by Prokaryotic Leucyl-tRNA Synthetase

	tRNA-dependent Pre-transfer Editing by Prokaryotic Leucyl-tRNA Synthetase
	Tan, M; Zhu, B; Zhou, XL; He, R; Chen, X; Eriani, G; Wang, ED
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2010
卷号	285 期号:5 页码:3235-3244
通讯作者	,edwang@sibs.ac.cn
英文摘要	To prevent genetic code ambiguity due to misincorporation of amino acids into proteins, aminoacyl-tRNA synthetases have evolved editing activities to eliminate intermediate or final non-cognate products. In this work we studied the different editing pathways of class Ia leucyl-tRNA synthetase (LeuRS). Different mutations and experimental conditions were used to decipher the editing mechanism, including the recently developed compound AN2690 that targets the post-transfer editing site of LeuRS. The study emphasizes the crucial importance of tRNA for the pre- and post-transfer editing catalysis. Both reactions have comparable efficiencies in prokaryotic Aquifex aeolicus and Escherichia coli LeuRSs, although the E. coli enzyme favors post-transfer editing, whereas the A. aeolicus enzyme favors pre-transfer editing. Our results also indicate that the entry of the CCA-acceptor end of tRNA in the editing domain is strictly required for tRNA-dependent pre-transfer editing. Surprisingly, this editing reaction was resistant to AN2690, which inactivates the enzyme by forming a covalent adduct with tRNALeu in the post-transfer editing site. Taken together, these data suggest that the binding of tRNA in the post-transfer editing conformation confers to the enzyme the capacity for pre-transfer editing catalysis, regardless of its capacity to catalyze post-transfer editing.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	MISACTIVATED AMINO-ACIDS ; ESCHERICHIA-COLI ; PROTEIN-SYNTHESIS ; AQUIFEX-AEOLICUS ; STRUCTURAL BASIS ; RATIONAL DESIGN ; ACTIVE-SITE ; ERRORS ; DISCRIMINATION ; TRANSLOCATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000273829000038
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1009]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Tan, M,Zhu, B,Zhou, XL,et al. tRNA-dependent Pre-transfer Editing by Prokaryotic Leucyl-tRNA Synthetase[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2010,285(5):3235-3244.
APA	Tan, M.,Zhu, B.,Zhou, XL.,He, R.,Chen, X.,...&Wang, ED.(2010).tRNA-dependent Pre-transfer Editing by Prokaryotic Leucyl-tRNA Synthetase.JOURNAL OF BIOLOGICAL CHEMISTRY,285(5),3235-3244.
MLA	Tan, M,et al."tRNA-dependent Pre-transfer Editing by Prokaryotic Leucyl-tRNA Synthetase".JOURNAL OF BIOLOGICAL CHEMISTRY 285.5(2010):3235-3244.