QSG-7701 Human Hepatocytes Form Polarized Acini in Three-Dimensional Culture

	QSG-7701 Human Hepatocytes Form Polarized Acini in Three-Dimensional Culture
	Zhang, F; Xu, R; Zhao, MJ
刊名	JOURNAL OF CELLULAR BIOCHEMISTRY
	2010
卷号	110 期号:5 页码:1175-1186
关键词	HEPATOCYTES THREE-DIMENSIONAL CULTURE MORPHOGENESIS POLARITY ACINUS BILE CANALICULUS
通讯作者	Zhao, MJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	Hepatocytes are polarized and fulfill a variety of liver-specific functions in vivo, but the polarized tissue structure and many of these functions are lost when the cells are cultured on plastic. To recapitulate the polarized structure and tissue-specific function of liver cells in culture, we established a three-dimensional (3D) culture assay with the human hepatocyte line QSG-7701. In 3D Matrigel culture, QSG-7701 cells formed polarized spheroids with a center lumen, which is reminiscent of bile canaliculi in the liver. Immunofluoresence analysis showed that F-actin bundles and radixin were mainly located at the apical membrane and that alpha 6 and beta 1 integrins were localized basally in 3D culture Lumen formation was associated with the selective apoptosis of centrally located cells and was accompanied by proliferative suppression during acmar development. Compared to QSG-7701 cells in 2D or agarose gel cultures, the cells in 3D Matrigel culture maintained a given direction of biliary excretion and acquired higher levels of cytochrome P450 and albumin expression Our study shows that the immortal human hepatocytes. QSG-7701, in 3D Matrigel culture reacquire cardinal features of glandular epithelium in vivo, providing an ex vivo model to study liver-specific function and tumorigenesis. J Cell Biochem. 110: 1175-1186, 2010 (C) 2010 Wiley-Liss. Inc
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	RAT HEPATOCYTES ; IN-VITRO ; CELLS ; LIVER ; EXPRESSION ; DIFFERENTIATION ; MAINTENANCE ; MATRIX ; CONFIGURATION ; ARCHITECTURE
收录类别	SCI
语种	英语
WOS记录号	WOS:000280435900016
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1003]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, F,Xu, R,Zhao, MJ. QSG-7701 Human Hepatocytes Form Polarized Acini in Three-Dimensional Culture[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2010,110(5):1175-1186.
APA	Zhang, F,Xu, R,&Zhao, MJ.(2010).QSG-7701 Human Hepatocytes Form Polarized Acini in Three-Dimensional Culture.JOURNAL OF CELLULAR BIOCHEMISTRY,110(5),1175-1186.
MLA	Zhang, F,et al."QSG-7701 Human Hepatocytes Form Polarized Acini in Three-Dimensional Culture".JOURNAL OF CELLULAR BIOCHEMISTRY 110.5(2010):1175-1186.