Low-density lipoprotein receptor-related protein 1 is an essential receptor for trichosanthin in 2 choriocarcinoma cell lines

	Low-density lipoprotein receptor-related protein 1 is an essential receptor for trichosanthin in 2 choriocarcinoma cell lines
	Jiao, YZ; Liu, WY
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2010
卷号	391 期号:4 页码:1579-1584
关键词	Trichosanthin Ribosome-inactivating protein Low-density lipoprotein receptor-related protein 1 Choriocarcinoma cells Receptor binding Endocytosis
通讯作者	Liu, WY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,wyliu@sibs.ac.cn
英文摘要	Type-I ribosome-inactivating protein-trichosanthin (TCS) exhibits selective cytotoxicity toward different types of cells. It is believed that the cytotoxicity results from the inhibition of ribosomes to decrease protein synthesis, thereby indicating that there are specific mechanisms for TCS entry into target cells to reach the ribosomes. Low-density lipoprotein (LDL) receptor-related protein 1 (LRP1) is a large scavenger receptor that is responsible for the binding and endocytosis of diverse biological ligands on the cell surface. In this study, we demonstrated that 2 choriocarcinoma cell lines can significantly bind and internalize TCS. In contrast, Hela cell line displayed no obvious TCS binding and endocytosis. Furthermore LRP1 gene silencing in JAR and BeWo cell lines blocked TCS binding: TCS could also interact with LRP1.The results of our study established that LRP1 was a major receptor for phagocytosis of TCS in JAR and BeWo cell lines and might be the molecular basis of TCS abortificient and anti-choriocarcinoma activity. (C) 2009 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	PSEUDOMONAS EXOTOXIN-A ; PLASMINOGEN-ACTIVATOR ; DEGRADATION ; CYTOTOXICITY ; PATHWAY ; CYTOSOL ; FAMILY ; ENTER ; RICIN ; LRP
收录类别	SCI
语种	英语
WOS记录号	WOS:000274097900001
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/998]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Jiao, YZ,Liu, WY. Low-density lipoprotein receptor-related protein 1 is an essential receptor for trichosanthin in 2 choriocarcinoma cell lines[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2010,391(4):1579-1584.
APA	Jiao, YZ,&Liu, WY.(2010).Low-density lipoprotein receptor-related protein 1 is an essential receptor for trichosanthin in 2 choriocarcinoma cell lines.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,391(4),1579-1584.
MLA	Jiao, YZ,et al."Low-density lipoprotein receptor-related protein 1 is an essential receptor for trichosanthin in 2 choriocarcinoma cell lines".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 391.4(2010):1579-1584.