High-coverage proteome analysis reveals the first insight of protein modification systems in the pathogenic spirochete Leptospira interrogans

	High-coverage proteome analysis reveals the first insight of protein modification systems in the pathogenic spirochete Leptospira interrogans
	Cao, XJ; Dai, J; Xu, H; Nie, S; Chang, X; Hu, BY; Sheng, QH; Wang, LS; Ning, ZB; Li, YX
刊名	CELL RESEARCH
	2010
卷号	20 期号:2 页码:197-210
关键词	Leptospira interrogans posttranslational modification eukaryotic-like evolutionary conservation
通讯作者	Zeng, R (reprint author), Chinese Acad Sci, Key Lab Syst Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,gpzhao@sibs.ac.cn ; zr@sibs.ac.cn
英文摘要	Leptospirosis is a widespread zoonotic disease caused by pathogenic spirochetes of the genus Leptospira that infects humans and a wide range of animals. By combining computational prediction and high-accuracy tandem mass spectra, we revised the genome annotation of Leptospira interrogans serovar Lai, a free-living pathogenic spirochete responsible for leptospirosis, providing substantial peptide evidence for novel genes and new gene boundaries. Subsequently, we presented a high-coverage proteome analysis of protein expression and multiple posttranslational modifications ( PTMs). Approximately 64.3% of the predicted L. interrogans proteins were cataloged by detecting 2 540 proteins. Meanwhile, a profile of multiple PTMs was concurrently established, containing in total 32 phosphorylated, 46 acetylated and 155 methylated proteins. The PTM systems in the serovar Lai show unique features. Unique eukaryotic-like features of L. interrogans protein modifications were demonstrated in both phosphorylation and arginine methylation. This systematic analysis provides not only comprehensive information of high-coverage protein expression and multiple modifications in prokaryotes but also a view suggesting that the evolutionarily primitive L. interrogans shares significant similarities in protein modification systems with eukaryotes.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	OUTER-MEMBRANE PROTEINS ; ANTI-SIGMA-FACTOR ; MASS-SPECTROMETRY ; BACILLUS-SUBTILIS ; GENE ANNOTATION ; SEROVAR LAI ; MYCOBACTERIUM-TUBERCULOSIS ; COG DATABASE ; GENOME ; PHOSPHOPROTEOME
收录类别	SCI
语种	英语
WOS记录号	WOS:000275816100011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/958]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Cao, XJ,Dai, J,Xu, H,et al. High-coverage proteome analysis reveals the first insight of protein modification systems in the pathogenic spirochete Leptospira interrogans[J]. CELL RESEARCH,2010,20(2):197-210.
APA	Cao, XJ.,Dai, J.,Xu, H.,Nie, S.,Chang, X.,...&Zeng, R.(2010).High-coverage proteome analysis reveals the first insight of protein modification systems in the pathogenic spirochete Leptospira interrogans.CELL RESEARCH,20(2),197-210.
MLA	Cao, XJ,et al."High-coverage proteome analysis reveals the first insight of protein modification systems in the pathogenic spirochete Leptospira interrogans".CELL RESEARCH 20.2(2010):197-210.