Identification of Serum Biomarkers for Colorectal Cancer Metastasis Using a Differential Secretome Approach

	Identification of Serum Biomarkers for Colorectal Cancer Metastasis Using a Differential Secretome Approach
	Xue, H; Li, BJ; Zhang, J; Wu, ML; Huang, Q; Wu, Q; Sheng, HQ; Wu, DD; Hu, JW; Lai, MD
刊名	JOURNAL OF PROTEOME RESEARCH
	2010
卷号	9 期号:1 页码:545-555
关键词	biomarker colorectal cancer label-free quantitation metastasis secretome
通讯作者	Lai, MD (reprint author), Zhejiang Univ, Sch Med, Dept Pathol & Pathophysiol, 188 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China.,lmp@zju.edu.cn
英文摘要	Lymph node metastasis is the major concern that causes death in colorectal cancers. However, biomarkers for cancer metastasis are still lacking. In this study, we applied an LC-MS/MS-based label-free quantitative proteomics approach to compare the differential secretome of a primary cell line SW480 and its lymph node metastatic cell line SW620 from the same colorectal cancer patient. We identified a total of 910 proteins from the conditioned media and 145 differential proteins between SW480 and SW620 (>1.5-fold change). The differential expression pattern of 6 candidate proteins was validated by Western blot analysis. Among them, trefoil factor 3 and growth/differentiation factor 15, two up-regulated proteins in SW620, were further analyzed in a large cohort of clinical tissue and serum samples. Sandwich ELISA assay showed that the serum levels of both proteins were significantly higher in lymph node metastatic colorectal cancers. Receiver operating characteristic curve analysis confirmed that serum trefoil factor 3 and growth/differentiation factor 15 could provide a discriminatory diagnostic test for predicting colorectal cancer metastasis. Immunohistochemical analysis also showed that the overexpression of trefoil factor 3 or growth/differentiation factor 15 in colorectal cancer was associated with lymph node metastatic behavior. This study showed an accurate, sensitive, and robust label-free quantitation approach for differential analysis of cancer secretome. The comparison of the cancer secretome in vitro is a feasible strategy to obtain valuable biomarkers for potential clinical application. Both trefoil factor 3 and growth/differentiation factor 15 could serve as potential biomarkers for the prediction of colorectal cancer metastasis.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods
关键词[WOS]	MACROPHAGE INHIBITORY CYTOKINE-1 ; INTESTINAL TREFOIL FACTOR ; TGF-BETA SUPERFAMILY ; FREE QUANTITATIVE PROTEOMICS ; TUMOR-DERIVED EXOSOMES ; HUMAN BREAST-CANCER ; PROSTATE-CANCER ; COLON-CANCER ; CELL-LINES ; MASS-SPECTROMETRY
收录类别	SCI
语种	英语
WOS记录号	WOS:000273267900051
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/933]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xue, H,Li, BJ,Zhang, J,et al. Identification of Serum Biomarkers for Colorectal Cancer Metastasis Using a Differential Secretome Approach[J]. JOURNAL OF PROTEOME RESEARCH,2010,9(1):545-555.
APA	Xue, H.,Li, BJ.,Zhang, J.,Wu, ML.,Huang, Q.,...&Lai, MD.(2010).Identification of Serum Biomarkers for Colorectal Cancer Metastasis Using a Differential Secretome Approach.JOURNAL OF PROTEOME RESEARCH,9(1),545-555.
MLA	Xue, H,et al."Identification of Serum Biomarkers for Colorectal Cancer Metastasis Using a Differential Secretome Approach".JOURNAL OF PROTEOME RESEARCH 9.1(2010):545-555.