Zac1 is a histone acetylation-regulated NF-kappa B suppressor that mediates histone deacetylase inhibitor-induced apoptosis

	Zac1 is a histone acetylation-regulated NF-kappa B suppressor that mediates histone deacetylase inhibitor-induced apoptosis
	Shu, G; Tang, Y; Zhou, Y; Wang, C; Song, JG
刊名	CELL DEATH AND DIFFERENTIATION
	2011
卷号	18 期号:12 页码:1825-1835
关键词	Zac1 NF-kappa B apoptosis histone acetylation epigenetic regulation
通讯作者	Song, JG (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 YueYang Rd, Shanghai 200031, Peoples R China.,jgsong@sibs.ac.cn
英文摘要	Histone deacetylase (HDAC) inhibitors are a class of promising anticancer reagents. They are able to induce apoptosis in embryonic carcinoma (EC) cells. However, the underlying mechanism remains poorly understood. Here we show that increased expression of zinc-finger protein regulator of apoptosis and cell-cycle arrest (Zac1) is implicated in HDAC inhibitor-induced apoptosis in F9 and P19 EC cells. By chromatin immunoprecipitation analysis we identified that increased Zac1 expression is mediated by histone acetylation of the Zac1 promoter region. Knockdown of Zac1 inhibited HDAC inhibitor-induced cell apoptosis. Moreover, HDAC inhibitors repressed nuclear factor-kappa B (NF-kappa B) activity, and this effect is abrogated by Zac1 knockdown. Consistently, Zac1 overexpression suppressed cellular NF-kappa B activity. Further investigation showed that Zac1 inhibits NF-kappa B activity by interacting with the C-terminus of the p65 subunit, which suppresses the phosphorylation of p65 at Ser468 and Ser536 residues. These results indicate that Zac1 is a histone acetylation-regulated suppressor of NF-kappa B, which is induced and implicated in HDAC inhibitor-mediated EC cell apoptosis. Cell Death and Differentiation (2011) 18, 1825-1835; doi:10.1038/cdd.2011.51; published online 6 May 2011
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	ZINC-FINGER PROTEIN ; TUMOR-SUPPRESSOR ; TRANSCRIPTIONAL COACTIVATOR ; MESENCHYMAL TRANSITION ; CARCINOMA-CELLS ; GENE ZAC ; CANCER ; ACTIVATION ; P65 ; PHOSPHORYLATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000296968000002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/874]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Shu, G,Tang, Y,Zhou, Y,et al. Zac1 is a histone acetylation-regulated NF-kappa B suppressor that mediates histone deacetylase inhibitor-induced apoptosis[J]. CELL DEATH AND DIFFERENTIATION,2011,18(12):1825-1835.
APA	Shu, G,Tang, Y,Zhou, Y,Wang, C,&Song, JG.(2011).Zac1 is a histone acetylation-regulated NF-kappa B suppressor that mediates histone deacetylase inhibitor-induced apoptosis.CELL DEATH AND DIFFERENTIATION,18(12),1825-1835.
MLA	Shu, G,et al."Zac1 is a histone acetylation-regulated NF-kappa B suppressor that mediates histone deacetylase inhibitor-induced apoptosis".CELL DEATH AND DIFFERENTIATION 18.12(2011):1825-1835.