Site-directed mutagenesis of aromatic residues in the carbohydrate-binding module of Bacillus endoglucanase EGA decreases enzyme thermostability

	Site-directed mutagenesis of aromatic residues in the carbohydrate-binding module of Bacillus endoglucanase EGA decreases enzyme thermostability
	Yin, QY; Teng, YG; Ding, M; Zhao, FK
刊名	BIOTECHNOLOGY LETTERS
	2011
卷号	33 期号:11 页码:2209-2216
关键词	Aromatic amino acid Carbohydrate-binding module Cellulase Hydrophobic interaction Site-directed mutagenesis Thermostability
通讯作者	Zhao, FK (reprint author), Chinese Acad Sci, Grad Sch, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,fkzhao@sibs.ac.cn
英文摘要	The endoglucanase, EGA, fromBacillus sp. AC-1 comprises a glycosyl hydrolase family-9 catalytic module (CM9) and a family-3 carbohydrate-binding module (CBM3). Seven aromatic residues were subjected to site-directed mutagenesis in both CBM3 and EGA to investigate their roles in enzyme thermostability. The complexes generated by mixing CBMY527G, CBMW532A, or CBMF592G with CM9 each lost their activities after 15 min at 45 degrees C, while the wild-type complex retained >70% activity after 2 h. The mutants EGAY527G, EGAW532A, and EGAF592G showed little activity after 15 min at 60 degrees C, whereas EGA remained 70% active after 2 h. Thus the residues Tyr(527), Trp(532), and Phe(592) contribute not only to CBM3-mediated stability of CM9 but also to EGA thermostability suggesting that hydrophobic interaction between the two modules, independent of covalent linkages, is important for enzyme thermostability.
学科主题	Biotechnology & Applied Microbiology
类目[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	SWISS-MODEL ; CELLULOSE ; XYLANASE ; DOMAINS
收录类别	SCI
语种	英语
WOS记录号	WOS:000297163000014
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/866]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yin, QY,Teng, YG,Ding, M,et al. Site-directed mutagenesis of aromatic residues in the carbohydrate-binding module of Bacillus endoglucanase EGA decreases enzyme thermostability[J]. BIOTECHNOLOGY LETTERS,2011,33(11):2209-2216.
APA	Yin, QY,Teng, YG,Ding, M,&Zhao, FK.(2011).Site-directed mutagenesis of aromatic residues in the carbohydrate-binding module of Bacillus endoglucanase EGA decreases enzyme thermostability.BIOTECHNOLOGY LETTERS,33(11),2209-2216.
MLA	Yin, QY,et al."Site-directed mutagenesis of aromatic residues in the carbohydrate-binding module of Bacillus endoglucanase EGA decreases enzyme thermostability".BIOTECHNOLOGY LETTERS 33.11(2011):2209-2216.