Mitogen-activated protein kinases ERK 1/2- and p38-GATA4 pathways mediate the Ang II-induced activation of FGF2 gene in neonatal rat cardiomyocytes

	Mitogen-activated protein kinases ERK 1/2- and p38-GATA4 pathways mediate the Ang II-induced activation of FGF2 gene in neonatal rat cardiomyocytes
	Tang, WJ; Wei, YZ; Le, K; Li, Z; Bao, YX; Gao, J; Zhang, FY; Cheng, SR; Liu, PQ
刊名	BIOCHEMICAL PHARMACOLOGY
	2011
卷号	81 期号:4 页码:518-525
关键词	FGF2 Promoter Transcription factor GATA4 Cardiac hypertrophy
通讯作者	Liu, PQ (reprint author), Sun Yat Sen Univ, Sch Pharmaceut Sci, Dept Pharmacol & Toxicol, Lab Pharmacol & Toxicol, 132 Wai Huan E Rd, Guangzhou 510006, Guangdong, Peoples R China.,liupq@mail.sysu.edu.cn
英文摘要	Several genes, including fibroblast growth factor 2 (FGF2), are up-regulated in the hypertrophic heart. However, the molecular mechanisms responsible for the angiotensin II (Ang II)-induced activation of FGF2 in cardiomyocyte hypertrophy are largely unknown. The purpose of this study was to determine the signaling cascades underlying the Ang II-induced transcriptional activation of FGF2 in neonatal rat cardiomyocytes. Real-time quantitative RT-PCR and Western blot showed that Ang II upregulates FGF2 expression and that these effects were attenuated by U0126 or SB203580, but not by SP600125. Deletion analyses revealed that the region between -845 and -666 is essential for Ang II-induced FGF2 promoter activity. The existence of an atypical GATA4-binding motif, located at position -752, was identified using electrophoretic mobility shift assay (EMSA). Using both EMSA and chromatin immunoprecipitation (ChIP) analyses, we also showed that Ang II increases binding of GATA4 to DNA, and that this effect is attenuated in the presence of U0126 or SB203580, but not in the presence of SP600125. GATA4 siRNA significantly reduced Ang II-induced FGF2 mRNA levels. Together, these results indicate that binding of GATA4 to DNA is increased by Ang II via extracellular signal-regulated protein kinase 1/2 (ERK 1/2) and p38 kinase, which increases FGF2 gene expression in neonatal rat cardiomyocytes. (C) 2010 Elsevier Inc. All rights reserved.
学科主题	Pharmacology & Pharmacy
类目[WOS]	Pharmacology & Pharmacy
关键词[WOS]	FIBROBLAST GROWTH FACTOR-2 ; HIGH-MOLECULAR-WEIGHT ; ANGIOTENSIN-II ; CARDIAC-HYPERTROPHY ; TRANSCRIPTION FACTOR ; MYOCYTES ; EXPRESSION ; GATA-4 ; HEART ; MORPHOGENESIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000286703300006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/786]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Tang, WJ,Wei, YZ,Le, K,et al. Mitogen-activated protein kinases ERK 1/2- and p38-GATA4 pathways mediate the Ang II-induced activation of FGF2 gene in neonatal rat cardiomyocytes[J]. BIOCHEMICAL PHARMACOLOGY,2011,81(4):518-525.
APA	Tang, WJ.,Wei, YZ.,Le, K.,Li, Z.,Bao, YX.,...&Liu, PQ.(2011).Mitogen-activated protein kinases ERK 1/2- and p38-GATA4 pathways mediate the Ang II-induced activation of FGF2 gene in neonatal rat cardiomyocytes.BIOCHEMICAL PHARMACOLOGY,81(4),518-525.
MLA	Tang, WJ,et al."Mitogen-activated protein kinases ERK 1/2- and p38-GATA4 pathways mediate the Ang II-induced activation of FGF2 gene in neonatal rat cardiomyocytes".BIOCHEMICAL PHARMACOLOGY 81.4(2011):518-525.