Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition

	Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition
	Huang, ZH; Wang, Y; Su, ZD; Geng, JG; Chen, YZ; Yuan, XB; He, C
刊名	JOURNAL OF CELL SCIENCE
	2011
卷号	124 期号:2 页码:186-197
关键词	Ca2+ Cofilin Migration Olfactory ensheathing cells RhoA Slit-2
通讯作者	Yuan, XB (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai 200031, Peoples R China.,yuanxb@ion.ac.cn ; chenghe@smmu.edu.cn
英文摘要	Olfactory ensheathing cells (OECs) migrate from the olfactory epithelium towards the olfactory bulb during development. However, the guidance mechanism for OEC migration remains a mystery. Here we show that migrating OECs expressed the receptor of the repulsive guidance factor Slit-2. A gradient of Slit-2 in front of cultured OECs first caused the collapse of the leading front, then the reversal of cell migration. These Slit-2 effects depended on the Ca2+ release from internal stores through inositol (1,4,5)-triphosphate receptor channels. Interestingly, in response to Slit-2 stimulation, collapse of the leading front required the activation of the F-actin severing protein cofilin in a Ca2+-dependent manner, whereas the subsequent reversal of the soma migration depended on the reversal of RhoA activity across the cell. Finally, the Slit-2-induced repulsion of cell migration was fully mimicked by co-application of inhibitors of F-actin polymerization and RhoA kinase. Our findings revealed Slit-2 as a repulsive guidance factor for OEC migration and an unexpected link between Ca2+ and cofilin signaling during Slit-2-triggered repulsion.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	ACTIN-FILAMENT TURNOVER ; LIM-KINASE ; GROWTH CONE ; NEURONAL MIGRATION ; SLINGSHOT PHOSPHATASE ; AXON GUIDANCE ; NEURITE OUTGROWTH ; FAMILY GTPASES ; SPINAL-CORD ; IN-VITRO
收录类别	SCI
语种	英语
WOS记录号	WOS:000285634000007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/757]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Huang, ZH,Wang, Y,Su, ZD,et al. Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition[J]. JOURNAL OF CELL SCIENCE,2011,124(2):186-197.
APA	Huang, ZH.,Wang, Y.,Su, ZD.,Geng, JG.,Chen, YZ.,...&He, C.(2011).Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition.JOURNAL OF CELL SCIENCE,124(2),186-197.
MLA	Huang, ZH,et al."Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition".JOURNAL OF CELL SCIENCE 124.2(2011):186-197.