JAK2/STAT3 Pathway Is Involved in the Early Stage of Adipogenesis Through Regulating C/EBP beta Transcription

	JAK2/STAT3 Pathway Is Involved in the Early Stage of Adipogenesis Through Regulating C/EBP beta Transcription
	Zhang, KM; Guo, W; Yang, Y; Wu, JR
刊名	JOURNAL OF CELLULAR BIOCHEMISTRY
	2011
卷号	112 期号:2 页码:488-497
关键词	JAK2 STAT3 C/EBP beta ADIPOGENESIS
通讯作者	Wu, JR (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,wujr@sibs.ac.cn
英文摘要	Signal transducer and activator of transcription 3 (STAT3) was reported to be involved in adipogenesis. However, the regulating mechanism of STAT3 remains unclear. The present results showed that STAT3 was activated within 2-h adipogenic induction, in which the phosphorylated STAT3 translocated from cytoplasm to the nucleus. In addition, we detected Janus kinase2 (JAK2) acted upstream of the STAT3 activation at the early stage of adipogenesis. Accordingly, the JAK2 inhibitor AG490 and siRNAs led to the partial inhibition of the STAT3 activation, and the inhibition of 3T3-L1 adipocyte differentiation. Furthermore, the results based on lueiferase, chromatin immunoprecipitation, and gel shift approaches indicated that STAT3 could regulate the transcription of C/EBP beta by binding the distal region of C/EBP beta promoter at the early stage of adipogenesis. Collectively, our findings reveal that JAK2/STAT3 pathway is involved in the early stage of 3T3-L1 adipocyte differentiation though regulating the C/EBP beta transcription. J. Cell. Biochem. 112: 488-497, 2011. (C) 2010 Wiley-Liss, Inc.
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	ADIPOSE CELL LINE ; ADIPOCYTE DIFFERENTIATION ; GENE-EXPRESSION ; SIGNAL TRANSDUCER ; 3T3-L1 CELLS ; PROTEIN ; STATS ; PROLIFERATION ; CONVERSION ; INHIBITOR
收录类别	SCI
语种	英语
WOS记录号	WOS:000287071900017
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/744]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, KM,Guo, W,Yang, Y,et al. JAK2/STAT3 Pathway Is Involved in the Early Stage of Adipogenesis Through Regulating C/EBP beta Transcription[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2011,112(2):488-497.
APA	Zhang, KM,Guo, W,Yang, Y,&Wu, JR.(2011).JAK2/STAT3 Pathway Is Involved in the Early Stage of Adipogenesis Through Regulating C/EBP beta Transcription.JOURNAL OF CELLULAR BIOCHEMISTRY,112(2),488-497.
MLA	Zhang, KM,et al."JAK2/STAT3 Pathway Is Involved in the Early Stage of Adipogenesis Through Regulating C/EBP beta Transcription".JOURNAL OF CELLULAR BIOCHEMISTRY 112.2(2011):488-497.