MCPIP1 Down-Regulates IL-2 Expression through an ARE-Independent Pathway

	MCPIP1 Down-Regulates IL-2 Expression through an ARE-Independent Pathway
	Li, M; Cao, WQ; Liu, HF; Zhang, W; Liu, X; Cai, ZJ; Guo, J; Wang, XL; Hui, ZY; Zhang, H
刊名	PLOS ONE
	2012
卷号	7 期号:11 页码:e49841-e49841
通讯作者	Wang, JL (reprint author), Zhejiang Univ, Sch Med, Inst Immunol, Hangzhou 310003, Zhejiang, Peoples R China.,jlwang@zju.edu.cn ; wanglie@zju.edu.cn
英文摘要	IL-2 plays a key role in the survival and proliferation of immune cells, especially T lymphocytes. Its expression is precisely regulated at transcriptional and posttranscriptional level. IL-2 is known to be regulated by RNA binding proteins, such as tristetraprolin (TTP), via an AU-rich element (ARE) in the 3'-untranslated region (3'UTR) to influence the stability of mRNA. MCPIP1, identified as a novel RNase, can degrade IL-6, IL-12 and TNF-alpha mRNA by an ARE-independent pathway in the activation of macrophages. Here, we reported that MCPIP1 was induced in the activation of T lymphocytes and negatively regulated IL-2 gene expression in both mouse and human primary T lymphocytes through destabilizing its mRNA. A set of Luciferase reporter assay demonstrated that a non-ARE conserved element in IL-2 3'UTR, which formed a stem-loop structure, responded to MCPIP1 activity. RNA immunoprecipitation and Biotin pulldown experiments further suggested that MCPIP1 could modestly bind to IL-2 mRNA. Taken together, these data demonstrate that MCPIP1 down-regulates IL-2 via an ARE-independent pathway.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	MESSENGER-RNA DECAY ; AU-RICH ELEMENTS ; TRANSCRIPTION FACTOR ; GENE-EXPRESSION ; TRISTETRAPROLIN ; DESTABILIZATION ; PROMOTES ; DEADENYLATION ; DEGRADATION ; ACTIVATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000311821000120
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/654]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, M,Cao, WQ,Liu, HF,et al. MCPIP1 Down-Regulates IL-2 Expression through an ARE-Independent Pathway[J]. PLOS ONE,2012,7(11):e49841-e49841.
APA	Li, M.,Cao, WQ.,Liu, HF.,Zhang, W.,Liu, X.,...&Wang, L.(2012).MCPIP1 Down-Regulates IL-2 Expression through an ARE-Independent Pathway.PLOS ONE,7(11),e49841-e49841.
MLA	Li, M,et al."MCPIP1 Down-Regulates IL-2 Expression through an ARE-Independent Pathway".PLOS ONE 7.11(2012):e49841-e49841.