Potent and Specific Antitumor Effect for Colorectal Cancer by CEA and Rb Double Regulated Oncolytic Adenovirus Harboring ST13 Gene

	Potent and Specific Antitumor Effect for Colorectal Cancer by CEA and Rb Double Regulated Oncolytic Adenovirus Harboring ST13 Gene
	Zhou, XM; Xie, GL; Wang, SB; Wang, YG; Zhang, KJ; Zheng, S; Chu, L; Xiao, LL; Yu, YM; Zhang, Y
刊名	PLOS ONE
	2012
卷号	7 期号:10 页码:e47566-e47566
通讯作者	Liu, XY (reprint author), Zhejiang Sci Tech Univ, Coll Life Sci, Xinyuan Inst Med & Biotechnol, Hangzhou, Zhejiang, Peoples R China.,xyliu@sibs.ac.cn
英文摘要	Cancer Targeting Gene-Viro-Therapy (CTGVT) is constructed by inserting an antitumor gene into an oncolytic virus (OV). It is actually an OV-gene therapy, which has much better antitumor effect than either gene therapy alone or virotherapy alone in our previously published papers. This study is a modification of CTGVT by inserting a colorectal cancer (CRC) specific suppressor gene, ST13, into a CRC specific oncolytic virus, the Ad.CEA.E1A(Delta 24), to construct the Ad.(ST13).CEA.E1A(Delta 24) for increasing the targeting tropism to colorectal cancer and it was briefly named as CTGVT-CRC. Although many studies on CEA promoter and ST13 gene were reported but no construct has been performed to combine both of them as a new strategy for colorectal cancer (CRC) specific therapy. In addition to the CRC specificity, the antitumor effect of Ad.(ST13).CEA.E1A(Delta 24) was also excellent and got nearly complete inhibition (not eradication) of CRC xenograft since ST13 was an effective antitumor gene with less toxicity, and a Chinese patent (No. 201110319434.4) was available for this study. Ad.(ST13).CEA.E1A(Delta 24) caused cell apoptosis through P38 MAPK (i.e. P38) which upregulated CHOP and ATF2 expression. The mitochondrial medicated apoptosis pathway was activated by the increase of caspase 9 and caspase 3 expression.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	APOPTOSIS-INDUCING LIGAND ; CARCINOEMBRYONIC ANTIGEN ; PANCREATIC-CANCER ; TUMOR-SUPPRESSOR ; VIRO-THERAPY ; COLON-CANCER ; TRAIL ; VIROTHERAPY ; CARCINOMA ; PROMOTER
收录类别	SCI
语种	英语
WOS记录号	WOS:000309995100134
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/648]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhou, XM,Xie, GL,Wang, SB,et al. Potent and Specific Antitumor Effect for Colorectal Cancer by CEA and Rb Double Regulated Oncolytic Adenovirus Harboring ST13 Gene[J]. PLOS ONE,2012,7(10):e47566-e47566.
APA	Zhou, XM.,Xie, GL.,Wang, SB.,Wang, YG.,Zhang, KJ.,...&Liu, XY.(2012).Potent and Specific Antitumor Effect for Colorectal Cancer by CEA and Rb Double Regulated Oncolytic Adenovirus Harboring ST13 Gene.PLOS ONE,7(10),e47566-e47566.
MLA	Zhou, XM,et al."Potent and Specific Antitumor Effect for Colorectal Cancer by CEA and Rb Double Regulated Oncolytic Adenovirus Harboring ST13 Gene".PLOS ONE 7.10(2012):e47566-e47566.