The C-terminal Helices of Heat Shock Protein 70 Are Essential for J-domain Binding and ATPase Activation

	The C-terminal Helices of Heat Shock Protein 70 Are Essential for J-domain Binding and ATPase Activation
	Gao, XC; Zhou, CJ; Zhou, ZR; Wu, M; Cao, CY; Hu, HY
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2012
卷号	287 期号:8 页码:6044-6052
通讯作者	Hu, HY (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,hyhu@sibs.ac.cn
英文摘要	The J-domain co-chaperones work together with the heat shock protein 70 (HSP70) chaperone to regulate many cellular events, but the mechanism underlying the J-domain-mediated HSP70 function remains elusive. We studied the interaction between human-inducible HSP70 and Homo sapiens J-domain protein (HSJ1a), a J domain and UIM motif-containing co-chaperone. The J domain of HSJ1a shares a conserved structure with other J domains from both eukaryotic and prokaryotic species, and it mediates the interaction with and the ATPase cycle of HSP70. Our in vitro study corroborates that the N terminus of HSP70 including the ATPase domain and the substrate-binding beta-subdomain is not sufficient to bind with the J domain of HSJ1a. The C-terminal helical alpha-subdomain of HSP70, which was considered to function as a lid of the substrate-binding domain, is crucial for binding with the J domain of HSJ1a and stimulating the ATPase activity of HSP70. These fluctuating helices are likely to contribute to a proper conformation of HSP70 for J-domain binding other than directly bind with the J domain. Our findings provide an alternative mechanism of allosteric activation for functional regulation of HSP70 by its J-domain co-chaperones.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	MOLECULAR CHAPERONE DNAK ; SUBSTRATE-BINDING ; HSP70 CHAPERONES ; ALPHA-SYNUCLEIN ; NMR STRUCTURE ; RICH REGION ; PROTEIN ; HSP40 ; COCHAPERONE ; DYNAMICS
收录类别	SCI
语种	英语
WOS记录号	WOS:000300638000077
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/636]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Gao, XC,Zhou, CJ,Zhou, ZR,et al. The C-terminal Helices of Heat Shock Protein 70 Are Essential for J-domain Binding and ATPase Activation[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2012,287(8):6044-6052.
APA	Gao, XC,Zhou, CJ,Zhou, ZR,Wu, M,Cao, CY,&Hu, HY.(2012).The C-terminal Helices of Heat Shock Protein 70 Are Essential for J-domain Binding and ATPase Activation.JOURNAL OF BIOLOGICAL CHEMISTRY,287(8),6044-6052.
MLA	Gao, XC,et al."The C-terminal Helices of Heat Shock Protein 70 Are Essential for J-domain Binding and ATPase Activation".JOURNAL OF BIOLOGICAL CHEMISTRY 287.8(2012):6044-6052.