Neuronal conditional knockout of NRSF decreases vulnerability to seizures induced by pentylenetetrazol in mice

	Neuronal conditional knockout of NRSF decreases vulnerability to seizures induced by pentylenetetrazol in mice
	Liu, M; Sheng, ZJ; Cai, L; Zhao, K; Tian, Y; Fei, J
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2012
卷号	44 期号:6 页码:476-482
关键词	neuron restrictive silencer factor conditional knockout pentylenetetrazol seizure
通讯作者	Fei, J (reprint author), Tongji Univ, Sch Life Sci & Technol, Shanghai 200092, Peoples R China.,jfei@tongji.edu.cn
英文摘要	Neuron restrictive silencer factor (NRSF), also known as repressor element-1 silencing transcription factor, has been reported to modulate neuronal excitability and acts as endogenous anticonvulsant in kainic acid-induced or kindling-evoked seizure activity. However, whether NRSF functions in pentylenetetrazol (PTZ)-induced seizure activity has never been studied. To investigate the role of endogenous NRSF in the epileptogenesis induced by PTZ, in our experiment, NRSF neuronal conditional knockout mice (NRSF cKO) were adopted, in which NRSF was specifically deleted in neurons by the Cre-loxP system. Seizure threshold for PTZ, including the dose-response convulsions and the threshold dose, was compared between NRSF cKO and control mice. The threshold dose of PTZ that induced clonic and tonic seizures was significantly higher in NRSF cKO mice compared with the control. Similarly, the median lethal dose (LD50) of PTZ in NRSF cKO mice was also considerably higher than that of the control mice. These results revealed that NRSF cKO mice are of higher resistance to convulsions induced by PTZ. Our work first demonstrated the function of NRSF in PTZ-induced seizure and provided new evidence for differential pathways in diverse types of seizure.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	RESTRICTIVE SILENCER FACTOR ; NEURAL-SPECIFIC EXPRESSION ; TARGET GENES ; HISTONE DEACETYLASE ; FACTOR REST/NRSF ; DOWN-SYNDROME ; IN-VIVO ; TRANSCRIPTION ; REST ; REPRESSOR
收录类别	SCI
语种	英语
WOS记录号	WOS:000304830700002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/613]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, M,Sheng, ZJ,Cai, L,et al. Neuronal conditional knockout of NRSF decreases vulnerability to seizures induced by pentylenetetrazol in mice[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2012,44(6):476-482.
APA	Liu, M,Sheng, ZJ,Cai, L,Zhao, K,Tian, Y,&Fei, J.(2012).Neuronal conditional knockout of NRSF decreases vulnerability to seizures induced by pentylenetetrazol in mice.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,44(6),476-482.
MLA	Liu, M,et al."Neuronal conditional knockout of NRSF decreases vulnerability to seizures induced by pentylenetetrazol in mice".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 44.6(2012):476-482.