Proteomic profiling of lipid droplet-associated proteins in primary adipocytes of normal and obese mouse

	Proteomic profiling of lipid droplet-associated proteins in primary adipocytes of normal and obese mouse
	Ding, YB; Wu, YB; Zeng, R; Liao, K
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2012
卷号	44 期号:5 页码:394-406
关键词	proteomics obesity lipid droplet-associated protein primary adipocyte
通讯作者	Liao, K (reprint author), Chinese Acad Sci, State Key Lab Cell Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci,Grad Sch, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn
英文摘要	Lipid droplets in adipocytes serve as the principal long-term energy storage depot of animals. There is increasing recognition that lipid droplets are not merely a static neutral lipid storage site, but in fact dynamic and multi-functional organelles. Structurally, lipid droplet consists of a neutral lipid core surrounded by a phospholipid monolayer and proteins embedded in or bound to the phospholipid layer. Proteins on the surface of lipid droplets are crucial to droplet structure and dynamics. To understand the lipid droplet-associated proteome of primary adipocyte with a large central lipid droplet, lipid droplets of white adipose tissue from C57BL/6 mice were isolated. And the proteins were extracted and analyzed by liquid chromatography coupled with tandem mass spectrometry. A total of 193 proteins including 73 previously unreported proteins were identified. Furthermore, the isotope-coded affinity tags (ICAT) was used to compare the difference of lipid droplet-associated proteomes between the normal lean and the high-fat diet-induced obese C57BL/6 mice. Of 23 proteins quantified by ICAT analysis, 3 proteins were up-regulated and 4 proteins were down-regulated in the lipid droplets of adipose tissue from the obese mice. Importantly, two structural proteins of lipid droplets, perilipin A and vimentin, were greatly reduced in the lipid droplets of the adipose tissue from the obese mice, implicating reduced protein machinery for lipid droplet stability.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	ENDOPLASMIC-RETICULUM ; ADIPOSE CONVERSION ; 3T3-L1 ADIPOCYTES ; SURFACE-LAYER ; PERILIPIN ; CELL ; MEMBRANE ; BODIES ; IDENTIFICATION ; LIPOLYSIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000303333800003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/600]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ding, YB,Wu, YB,Zeng, R,et al. Proteomic profiling of lipid droplet-associated proteins in primary adipocytes of normal and obese mouse[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2012,44(5):394-406.
APA	Ding, YB,Wu, YB,Zeng, R,&Liao, K.(2012).Proteomic profiling of lipid droplet-associated proteins in primary adipocytes of normal and obese mouse.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,44(5),394-406.
MLA	Ding, YB,et al."Proteomic profiling of lipid droplet-associated proteins in primary adipocytes of normal and obese mouse".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 44.5(2012):394-406.