Secretome-Derived Isotope Tags (SDIT) Reveal Adipocyte-Derived Apolipoprotein C-I as a Predictive Marker for Cardiovascular Disease

	Secretome-Derived Isotope Tags (SDIT) Reveal Adipocyte-Derived Apolipoprotein C-I as a Predictive Marker for Cardiovascular Disease
	Li, RX; Ding, YB; Zhao, SL; Xiao, YY; Li, QR; Xia, FY; Sun, L; Lin, X; Wu, JR; Liao, K
刊名	JOURNAL OF PROTEOME RESEARCH
	2012
卷号	11 期号:5 页码:2851-2862
关键词	secretome-derived isotope tags adipocyte-secreted proteins obesity apolipoprotein C-I cardiovascular risk factors
通讯作者	Zeng, R (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Syst Biol, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn ; zr@sibs.ac.cn
英文摘要	We developed a quantitative strategy, named secretome-derived isotopic tag (SDIT), to concurrently identify and quantify the adipocyte-secreted plasma proteins from normal and high-fat-diet (HFD) induced obese mice, based on the application of isotope-labeled secreted proteins from cultured mouse adipocytes as internal standards. We detected 197 proteins with significant changes between normal and obese mice plasma. Importantly, a novel adipocyte-secreted plasma protein, apolipoprotein C-I (apoC-I), significantly increased in the obese mice plasma. The expression and secretion of adipocyte apoC-I was detected in differentiated 3T3-L1 and primary rat adipocytes. Our in vitro experiments proved that functional Golgi apparatus was required for apoC-I secretion. Additionally, obese mice had increased apoC-I production in adipose tissue. Population survey of 367 participants showed that the plasma level of apoC-I was significantly increased in obese individuals compared with healthy individuals. After multiple adjustments for age and sex, the odds ratios for risk factors of cardiovascular disease including high LDL cholesterol, hypercholesterolemia, and hypertriglyceridemia, respectively, were used to compare the highest with the lowest apoC-I quartile. Taken together, our studies provide a novel strategy to concurrently identify and quantify tissue-specific secreted proteins. This strategy can be used to identify the largest global characterization of adipocyte-derived plasma proteome and provides a potential disease-related biomarker for clinical diagnoses. By selectively analyzing adipocyte-secreted proteins in plasma from obese vs lean murine and/or human subjects, we discovered that apoC-I is an adipocyte-secreted plasma protein and a predictive marker for cardiovascular disease.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods
关键词[WOS]	ESTER TRANSFER PROTEIN ; ADIPOSE-TISSUE ; APOC-I ; METABOLIC SYNDROME ; MASS-SPECTROMETRY ; SEVERE HYPERTRIGLYCERIDEMIA ; LINKAGE DISEQUILIBRIUM ; TRANSGENIC MICE ; PROTEOMICS ; DIFFERENTIATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000303492100019
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/595]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, RX,Ding, YB,Zhao, SL,et al. Secretome-Derived Isotope Tags (SDIT) Reveal Adipocyte-Derived Apolipoprotein C-I as a Predictive Marker for Cardiovascular Disease[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(5):2851-2862.
APA	Li, RX.,Ding, YB.,Zhao, SL.,Xiao, YY.,Li, QR.,...&Zeng, R.(2012).Secretome-Derived Isotope Tags (SDIT) Reveal Adipocyte-Derived Apolipoprotein C-I as a Predictive Marker for Cardiovascular Disease.JOURNAL OF PROTEOME RESEARCH,11(5),2851-2862.
MLA	Li, RX,et al."Secretome-Derived Isotope Tags (SDIT) Reveal Adipocyte-Derived Apolipoprotein C-I as a Predictive Marker for Cardiovascular Disease".JOURNAL OF PROTEOME RESEARCH 11.5(2012):2851-2862.