SAP suppresses the development of experimental autoimmune encephalomyelitis in C57BL/6 mice

	SAP suppresses the development of experimental autoimmune encephalomyelitis in C57BL/6 mice
	Ji, Z; Ke, ZJ; Geng, JG
刊名	IMMUNOLOGY AND CELL BIOLOGY
	2012
卷号	90 期号:4 页码:388-395
关键词	experimental autoimmune encephalomyelitis (EAE) serum amyloid P component (SAP) P-Selectin
通讯作者	Geng, JG (reprint author), Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,jizhe2008@gmail.com
英文摘要	Experimental autoimmune encephalomyelitis (EAE) is a CD4(+) T cell-mediated disease of the central nervous system. Serum amyloid P component (SAP) is a highly conserved plasma protein named for its universal presence in amyloid deposits. Here we report that SAP-transgenic mice had unexpectedly attenuated EAE due to impaired encephalitogenic responses. Following induction with myelin oligodendroglial glycoprotein (MOG) peptide 35-55 in complete Freund's adjuvant, SAP-transgenic mice showed reduced spinal cord inflammation with lower severity of EAE attacks as compared with control C57BL/6 mice. However, in SAP-Knockout mice, the severity of EAE is enhanced. Adoptive transfer of Ag-restimulated T cells from wild type to SAP-transgenic mice, or transfer of SAP-transgenic Ag-restimulated T cells to control mice, induced milder EAE. T cells from MOG-primed SAP-transgenic mice showed weak proliferative responses. Furthermore, in SAP-transgenic mice, there is little infiltration of CD45-positive cells in the spinal cord. In vitro, SAP suppressed the secretion of interleukin-2 stimulated by P-selectin and blocked P-selectin binding to T cells. Moreover, SAP could change the affinity between alpha 4-integrin and T cells. These data suggested that SAP could antagonize the development of the acute phase of inflammation accompanying EAE by modulating the function of P-selectin. Immunology and Cell Biology (2012) 90, 388-395; doi:10.1038/icb.2011.51; published online 7 June 2011
学科主题	Cell Biology; Immunology
类目[WOS]	Cell Biology ; Immunology
关键词[WOS]	AMYLOID-P-COMPONENT ; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS ; CENTRAL-NERVOUS-SYSTEM ; C-REACTIVE PROTEIN ; SELECTIN GLYCOPROTEIN LIGAND-1 ; NF-KAPPA-B ; ALPHA-4 INTEGRIN ; HUMAN SERUM ; T-CELLS ; LEUKOCYTE RECRUITMENT
收录类别	SCI
语种	英语
WOS记录号	WOS:000303002900005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/576]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ji, Z,Ke, ZJ,Geng, JG. SAP suppresses the development of experimental autoimmune encephalomyelitis in C57BL/6 mice[J]. IMMUNOLOGY AND CELL BIOLOGY,2012,90(4):388-395.
APA	Ji, Z,Ke, ZJ,&Geng, JG.(2012).SAP suppresses the development of experimental autoimmune encephalomyelitis in C57BL/6 mice.IMMUNOLOGY AND CELL BIOLOGY,90(4),388-395.
MLA	Ji, Z,et al."SAP suppresses the development of experimental autoimmune encephalomyelitis in C57BL/6 mice".IMMUNOLOGY AND CELL BIOLOGY 90.4(2012):388-395.