pVHL Mediates K63-Linked Ubiquitination of nCLU

	pVHL Mediates K63-Linked Ubiquitination of nCLU
	Xue, J; Lv, DD; Jiao, S; Zhao, WT; Li, XB; Sun, H; Yan, B; Fan, L; Hu, RG; Fang, J
刊名	PLOS ONE
	2012
卷号	7 期号:4 页码:e35848-e35848
通讯作者	Fang, J (reprint author), Chinese Acad Sci, SIBS, Inst Nutr Sci, Key Lab Nutr & Metab, Shanghai, Peoples R China.,jfang@sibs.ac.cn
英文摘要	pVHL, product of von Hippel-Lindau (VHL) tumor suppressor gene, functions as the substrate recognition component of an E3-ubiquitin ligase that targets proteins for ubiquitination and proteasomal degradation. Hypoxia-inducible factor alpha (HIF alpha) is the well-known substrate of pVHL. Besides HIF alpha, pVHL also binds to many other proteins and has multiple functions. In this manuscript, we report that the nuclear clusterin (nCLU) is a target of pVHL. We found that pVHL had a direct interaction with nCLU. nCLU bound to pVHL at pVHL's beta domain, the site for recognition of substrate, indicating that nCLU might be a substrate of pVHL. Interestingly, pVHL bound to nCLU but did not lead to nCLU destruction. Further studies indicated that pVHL mediated K63-linked ubiquitination of nCLU and promoted nCLU nuclear translocation. In summary, our results disclose a novel function of pVHL that mediates K63-linked ubiquitination and identify nCLU as a new target of pVHL.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	LINDAU TUMOR-SUPPRESSOR ; PROLINE HYDROXYLATION ; PROTEIN-KINASE ; CANCER CELLS ; HIF-ALPHA ; CLUSTERIN ; COMPLEX ; LIGASE ; UBIQUITYLATION ; ACTIVATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000305339200118
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/570]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xue, J,Lv, DD,Jiao, S,et al. pVHL Mediates K63-Linked Ubiquitination of nCLU[J]. PLOS ONE,2012,7(4):e35848-e35848.
APA	Xue, J.,Lv, DD.,Jiao, S.,Zhao, WT.,Li, XB.,...&Fang, J.(2012).pVHL Mediates K63-Linked Ubiquitination of nCLU.PLOS ONE,7(4),e35848-e35848.
MLA	Xue, J,et al."pVHL Mediates K63-Linked Ubiquitination of nCLU".PLOS ONE 7.4(2012):e35848-e35848.