Structural insight into the mutual recognition and regulation between Suppressor of Fused and Gli/Ci

	Structural insight into the mutual recognition and regulation between Suppressor of Fused and Gli/Ci
	Zhang, Y; Fu, L; Qi, XL; Zhang, ZY; Xia, YX; Jia, JH; Jiang, J; Zhao, Y; Wu, G
刊名	NATURE COMMUNICATIONS
	2013
卷号	4 期号:1 页码:42006
通讯作者	Zhao, Y (reprint author), Chinese Acad Sci, State Key Lab Cell Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,yunzhao@sibcb.ac.cn ; geng.wu@sjtu.edu.cn
英文摘要	Hedgehog (Hh) signalling regulates embryonic development and adult tissue homoeostasis. Mutations of its pathway components including Suppressor of Fused (Sufu) and Gli/Ci predispose to cancers and congenital anomalies. The Sufu-Gli protein complex occupies a central position in the vertebrate Hh signalling pathway, especially in mammals. Here structures of full-length human and Drosophila Sufu, the human Sufu-Gli complex, along with normal mode analysis and FRET measurement results, reveal that Sufu alternates between 'open' and 'closed' conformations. The 'closed' form of Sufu is stabilized by Gli binding and inhibited by Hh treatment, whereas the 'open' state of Sufu is promoted by Gli-dissociation and Hh signalling. Mutations of critical interface residues disrupt the Sufu-Gli complex and prevent Sufu from repressing Gli-mediated transcription, tethering Gli in the cytoplasm and protecting Gli from the 26S proteasome-mediated degradation. Our study thus provides mechanistic insight into the mutual recognition and regulation between Sufu and Gli/Ci.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	HEDGEHOG SIGNAL-TRANSDUCTION ; CUBITUS-INTERRUPTUS ; NUCLEAR TRANSLOCATION ; TRANSCRIPTION FACTOR ; NEGATIVE REGULATOR ; SUFU ; PROTEINS ; DROSOPHILA ; MECHANISM ; FAMILY
收录类别	SCI
语种	英语
WOS记录号	WOS:000327948000001
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/499]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, Y,Fu, L,Qi, XL,et al. Structural insight into the mutual recognition and regulation between Suppressor of Fused and Gli/Ci[J]. NATURE COMMUNICATIONS,2013,4(1):42006.
APA	Zhang, Y.,Fu, L.,Qi, XL.,Zhang, ZY.,Xia, YX.,...&Wu, G.(2013).Structural insight into the mutual recognition and regulation between Suppressor of Fused and Gli/Ci.NATURE COMMUNICATIONS,4(1),42006.
MLA	Zhang, Y,et al."Structural insight into the mutual recognition and regulation between Suppressor of Fused and Gli/Ci".NATURE COMMUNICATIONS 4.1(2013):42006.