Identification of complex relationship between protein kinases and substrates during the cell cycle of HeLa cells by phosphoproteomic analysis

	Identification of complex relationship between protein kinases and substrates during the cell cycle of HeLa cells by phosphoproteomic analysis
	Yang, XL; Li, QR; Ning, ZB; Zhang, Y; Zeng, R; Wu, JR
刊名	PROTEOMICS
	2013
卷号	13 期号:8 页码:1233-1246
关键词	Cell cycle Kinase Network Quantitative phosphoproteome SILAC Systems biology
通讯作者	Zhang, Y (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Syst Biol, Shanghai 20031, Peoples R China.,yanzhang01@sibs.ac.cn
英文摘要	Each phase of eukaryotic cell cycle is tightly controlled by multicomponent regulatory networks based on complex relationships of protein phosphorylation. In order to better understand the relationships between kinases and their substrate proteins during the progression of cell cycle, we analyzed phosphoproteome of HeLa cells during G1, S, and G2/M phases of cell cycle using our developed quantitative phosphoproteomic approaches. A total of 4776 high-confidence phosphorylation sites (phosphosites) in 1177 proteins were identified. Bioinformatics analysis for predicting kinase groups revealed that 46 kinase groups could be assigned to 4321 phosphosites. The majority of phosphoproteins harboring two or more phosphosites could be phosphorylated by different kinase groups, in which nine major kinase groups accounted for more than 90% phosphosites. Further analyses showed that approximately half of the examined two phosphosite combinations were correlatively regulated, regardless of whether the kinase groups were same or not. In general, the majority of proteins containing correlated phosphosites had solely co-regulated or counter-regulated phosphosites, and co-regulation was significantly more frequent than counter-regulation, suggesting that the former may be more important for regulating the cell cycle. In conclusion, our findings provide new insights into the complex regulatory mechanisms of protein phosphorylation networks during eukaryotic cell cycle.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology
关键词[WOS]	MASS-SPECTROMETRY ; QUANTITATIVE PHOSPHOPROTEOMICS ; HIGH-RESOLUTION ; CONTINUOUS PH ; AMINO-ACIDS ; PHOSPHORYLATION ; CK2 ; PHOSPHOPEPTIDES ; EXPRESSION ; ENRICHMENT
收录类别	SCI
语种	英语
WOS记录号	WOS:000317684800003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/420]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yang, XL,Li, QR,Ning, ZB,et al. Identification of complex relationship between protein kinases and substrates during the cell cycle of HeLa cells by phosphoproteomic analysis[J]. PROTEOMICS,2013,13(8):1233-1246.
APA	Yang, XL,Li, QR,Ning, ZB,Zhang, Y,Zeng, R,&Wu, JR.(2013).Identification of complex relationship between protein kinases and substrates during the cell cycle of HeLa cells by phosphoproteomic analysis.PROTEOMICS,13(8),1233-1246.
MLA	Yang, XL,et al."Identification of complex relationship between protein kinases and substrates during the cell cycle of HeLa cells by phosphoproteomic analysis".PROTEOMICS 13.8(2013):1233-1246.