Lcn5 Promoter Directs the Region-Specific Expression of Cre Recombinase in Caput Epididymidis of Transgenic Mice

	Lcn5 Promoter Directs the Region-Specific Expression of Cre Recombinase in Caput Epididymidis of Transgenic Mice
	Xie, SS; Xu, J; Ma, WB; Liu, Q; Han, JX; Yao, GX; Huang, XX; Zhang, YL
刊名	BIOLOGY OF REPRODUCTION
	2013
卷号	88 期号:3 页码:71-71
关键词	caput Cre recombinase epididymis Lcn5 promoter male reproductive tract transgenic/knockout model
通讯作者	Zhang, YL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xingxuhuang@mail.nju.edu.cn ; ylzhang@sibs.ac.cn
英文摘要	Epididymis plays a crucial role in regulating the development of sperm motility and fertilizing capacity. To study the function of genes in the caput epididymidis using the Cre/loxP system, we generated Lcn5-Cre transgenic mice in which the expression of Cre recombinase is driven by the 1.8-kb Lcn5 promoter. A total of 11 founder mice carrying the Lcn5-Cre transgene were identified by PCR from 38 offspring, and the integration efficiency was 28.9%. However, only 1 of the 11 transgenic mouse lines were revealed with the Cre recombinase expressed specifically in caput epididymidis. Furthermore, expression of Cre mRNA was first observed on Postnatal Day 30 and continued to increase during development. Subsequently, Cre protein distribution was assessed by crossing Lcn5-Cre transgenic mice into the mT/mG reporter line. As expected, the Cre recombinase activity was only found in principal cells of the middle/distal caput epididymidis. The tissue-specific expression of Cre protein in the caput epididymidis was further confirmed using Lcn5-Cre mice crossed with a mouse strain carrying Aip1 conditional alleles (Aip1(flox/+)). In summary, a transgenic mouse line expressing Cre recombinase in principal cells of caput epididymidis was established. This transgenic mouse line can be used to generate conditional, caput epididymidis-specific knockout mouse models by crossing with mice harboring floxed (LoxP flanked) genes.
学科主题	Reproductive Biology
类目[WOS]	Reproductive Biology
关键词[WOS]	BINDING PROTEIN GENE ; MOUSE EPIDIDYMIS ; MALE-FERTILITY ; IN-VIVO ; CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000317471100012
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/351]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xie, SS,Xu, J,Ma, WB,et al. Lcn5 Promoter Directs the Region-Specific Expression of Cre Recombinase in Caput Epididymidis of Transgenic Mice[J]. BIOLOGY OF REPRODUCTION,2013,88(3):71-71.
APA	Xie, SS.,Xu, J.,Ma, WB.,Liu, Q.,Han, JX.,...&Zhang, YL.(2013).Lcn5 Promoter Directs the Region-Specific Expression of Cre Recombinase in Caput Epididymidis of Transgenic Mice.BIOLOGY OF REPRODUCTION,88(3),71-71.
MLA	Xie, SS,et al."Lcn5 Promoter Directs the Region-Specific Expression of Cre Recombinase in Caput Epididymidis of Transgenic Mice".BIOLOGY OF REPRODUCTION 88.3(2013):71-71.