Aly and THO are required for assembly of the human TREX complex and association of TREX components with the spliced mRNA

	Aly and THO are required for assembly of the human TREX complex and association of TREX components with the spliced mRNA
	Chi, BK; Wang, QL; Wu, GF; Tan, M; Wang, LT; Shi, M; Chang, XY; Cheng, H
刊名	NUCLEIC ACIDS RESEARCH
	2013
卷号	41 期号:2 页码:1294-1306
通讯作者	Cheng, H (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,hcheng@sibs.ac.cn
英文摘要	The mRNA export complex TREX (TREX) is known to contain Aly, UAP56, Tex1 and the THO complex, among which UAP56 is required for TREX assembly. Here, we systematically investigated the role of each human TREX component in TREX assembly and its association with the mRNA. We found that Tex1 is essentially a subunit of the THO complex. Aly, THO and UAP56 are all required for assembly of TREX, in which Aly directly interacts with THO subunits Thoc2 and Thoc5. Both Aly and THO function in linking UAP56 to the cap-binding protein CBP80. Interestingly, association of UAP56 with the spliced mRNA, but not with the pre-mRNA, requires Aly and THO. Unexpectedly, we found that Aly and THO require each other to associate with the spliced mRNA. Consistent with these biochemical results, similar to Aly and UAP56, THO plays critical roles in mRNA export. Together, we propose that Aly, THO and UAP56 form a highly integrated unit to associate with the spliced mRNA and function in mRNA export.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	COTRANSCRIPTIONAL RECRUITMENT ; TRANSCRIPTIONAL ELONGATION ; U2 SNRNP ; IN-VIVO ; EXPORT ; PROTEINS ; UAP56 ; ATP ; INTRONLESS ; GENES
收录类别	SCI
语种	英语
WOS记录号	WOS:000314121100056
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/349]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Chi, BK,Wang, QL,Wu, GF,et al. Aly and THO are required for assembly of the human TREX complex and association of TREX components with the spliced mRNA[J]. NUCLEIC ACIDS RESEARCH,2013,41(2):1294-1306.
APA	Chi, BK.,Wang, QL.,Wu, GF.,Tan, M.,Wang, LT.,...&Cheng, H.(2013).Aly and THO are required for assembly of the human TREX complex and association of TREX components with the spliced mRNA.NUCLEIC ACIDS RESEARCH,41(2),1294-1306.
MLA	Chi, BK,et al."Aly and THO are required for assembly of the human TREX complex and association of TREX components with the spliced mRNA".NUCLEIC ACIDS RESEARCH 41.2(2013):1294-1306.