Atypical PKC, regulated by Rho GTPases and Mek/Erk, phosphorylates Ezrin during eight-cell embryo compaction

	Atypical PKC, regulated by Rho GTPases and Mek/Erk, phosphorylates Ezrin during eight-cell embryo compaction
	Liu, HJ; Wu, ZT; Shi, XL; Li, WZ; Liu, C; Wang, DK; Ye, XY; Liu, L; Na, J; Cheng, H
刊名	DEVELOPMENTAL BIOLOGY
	2013
卷号	375 期号:1 页码:13-22
关键词	Ezrin phosphorylation Embryo compaction Mek/Erk PKC Rho GTPase
通讯作者	Chen, LY (reprint author), Nankai Univ, Collaborat Innovat Ctr Tianjin Med Epigenet 2011, State Key Lab Med Chem Biol, 94 Weijin Rd, Tianjin 300071, Peoples R China.,jie.na@tsinghua.edu.cn ; hcheng@sibs.ac.cn ; lingyichen@nankai.edu.cn
英文摘要	Phosphorylation of Ezrin T567 plays an important role in eight-cell embryo compaction. Yet, it is not clear how Ezrin phosphorylation is regulated during embryo compaction. Here, we demonstrated that inhibition of Mek/Erk or protein kinase C (PKC) signaling reduced the phosphorylation level of Ezrin T567 in eight-cell compacted embryos. Interestingly, the Rho GTPase inhibitor C3-transferase caused basolateral enrichment of atypical PKC (aPKC), as well as basolateral shift of phosphorylated Ezrin, suggesting aPKC may be a key regulator of Ezrin phosphorylation. Moreover, inhibition of PKC, but not Mek/Erk or Rho GTPases, affected the maintenance of Ezrin phosphorylation in compacted embryos. We further identified that aPKC is indeed required for Ezrin phosphorylation in eight-cell embryos. Taken together, Rho GTPases facilitate the apical distribution of aPKC and Ezrin. Subsequently, aPKC and Mek/Erk work together to promote Ezrin phosphorylation at the apical region, which in turn mediates the apical enrichment of filamentous actin, stabilizing the polarized apical region and allowing embryo compaction. Our data also suggested that aPKC might be the Ezrin kinase during eight-cell embryo compaction. (C) 2013 Elsevier Inc. All rights reserved.
学科主题	Developmental Biology
类目[WOS]	Developmental Biology
关键词[WOS]	MOUSE PREIMPLANTATION DEVELOPMENT ; ERM PROTEINS ; CELL-ADHESION ; MICROVILLAR BREAKDOWN ; CRUCIAL ROLE ; KINASE ; STAGE ; EMBRYOGENESIS ; BLASTOCYST ; MORPHOGENESIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000315479200002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/315]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, HJ,Wu, ZT,Shi, XL,et al. Atypical PKC, regulated by Rho GTPases and Mek/Erk, phosphorylates Ezrin during eight-cell embryo compaction[J]. DEVELOPMENTAL BIOLOGY,2013,375(1):13-22.
APA	Liu, HJ.,Wu, ZT.,Shi, XL.,Li, WZ.,Liu, C.,...&Chen, LY.(2013).Atypical PKC, regulated by Rho GTPases and Mek/Erk, phosphorylates Ezrin during eight-cell embryo compaction.DEVELOPMENTAL BIOLOGY,375(1),13-22.
MLA	Liu, HJ,et al."Atypical PKC, regulated by Rho GTPases and Mek/Erk, phosphorylates Ezrin during eight-cell embryo compaction".DEVELOPMENTAL BIOLOGY 375.1(2013):13-22.