miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis

	miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis
	Wang, DT; Ma, ZL; Li, YL; Wang, YQ; Zhao, BT; Wei, JL; Qiu, X; Zhao, XT; Jin, YX
刊名	ONCOLOGY REPORTS
	2013
卷号	30 期号:1 页码:492-498
关键词	miR-150 p53 protein miRNA NSCLC
通讯作者	Ma, ZL (reprint author), Shanghai Univ, Sch Life Sci, 99 Shangda Rd, Shanghai 200444, Peoples R China.,zlma@shu.edu.cn ; yxjin@sibs.ac.cn
英文摘要	microRNAs (miRNAs) are a class of non-coding small RNAs that act as negative regulators of gene expression by binding to the 3'-untranslated region (3'-UTR) of target mRNAs. Tumor protein p53, a transcriptional factor, plays an important role in the progression of tumorigenesis. miR-150 was the only miRNA predicted to target 3'-UTR of p53 by TargetScan. In order to investigate the function of miR-150, p53 and relevant miRNAs in non-small cell lung cancer (NSCLC), we constructed two expression vectors of p53 (pcDNA3.1-p53 and pcDNA3.1-p53-3'-UTR) and two report vectors (pGL3-p53-3'-UTR and pGL3-p53-3'-mUTR). The activity of luciferase transfected with miR-150 mimics was lower by 30% when compared to that of the miRNA-negative control (miRNA-NC). Moreover, the p53 protein was downregulated by at least 50% when miR-150 mimics were cotransfected with pcDNA3.1-p53-3'-UTR when compared to miRNA-NC. We also determined the expression of miR-150 and p53 in NSCLC patient tissue samples. The expression of miR-150 in T2 stage tissue samples was higher than that in T1 stage tissue samples. The corresponding target gene p53 was correlated with miR-150 expression. In the present study, we further analyzed the cell cycle distribution. The cells transfected with pcDNA3.1-p53 were significantly arrested in the G1 phase when compared to the control cells. When miR-150 mimics were cotransfected with pcDNA3.1-p53-3'-UTR, the percentage of cells in the G1 phase was significantly lower by 4% when compared to miRNA-NC. To identify miRNAs that are regulated by the p53 protein, qRT-PCR was performed after pcDNA3.1-p53 transfection. miR-34a, miR-184, miR-181a and miR-148 were upregulated significantly. However, there was no distinct difference in the expression of miR-10a, miR-182 and miR-34c. Our results showed that miR-150 targets the 3'-UTR of p53, and p53 protein promotes the expression of miRNAs which affect cell cycle progression. These findings suggest that miR-150, p53 protein and relevant miRNAs are members of a regulatory network in NSCLC tumorigenesis.
学科主题	Oncology
类目[WOS]	Oncology
关键词[WOS]	TARGETING NOTCH1 ; CANCER CELLS ; EXPRESSION ; PATHWAY ; MIR-34A ; PROLIFERATION ; MICRORNA-34A ; CARCINOMA ; APOPTOSIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000321077500069
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/312]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, DT,Ma, ZL,Li, YL,et al. miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis[J]. ONCOLOGY REPORTS,2013,30(1):492-498.
APA	Wang, DT.,Ma, ZL.,Li, YL.,Wang, YQ.,Zhao, BT.,...&Jin, YX.(2013).miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis.ONCOLOGY REPORTS,30(1),492-498.
MLA	Wang, DT,et al."miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis".ONCOLOGY REPORTS 30.1(2013):492-498.