Lithocholic acid-induced placental tumor necrosis factor-alpha upregulation and syncytiotrophoblast cell apoptosis in intrahepatic cholestasis of pregnancy

	Lithocholic acid-induced placental tumor necrosis factor-alpha upregulation and syncytiotrophoblast cell apoptosis in intrahepatic cholestasis of pregnancy
	Du, QL; Zhang, YH; Pan, YD; Duan, T
刊名	HEPATOLOGY RESEARCH
	2014
卷号	44 期号:5 页码:532-541
关键词	bile acid tumor necrosis factor-alpha intrahepatic cholestasis of pregnancy lithocholic acid syncytiotrophoblast
通讯作者	Duan, T (reprint author), Tongji Univ, Sch Med, Dept Obstet, Shanghai Matern & Infant Hosp 1, Shanghai 200040, Peoples R China.,ydpan@sibcb.ac.cn ; qldu2006@yahoo.cn
英文摘要	Aim To investigate tumor necrosis factor (TNF)-alpha expression and its relationship with serum bile acids in placental trophoblasts from patients with intrahepatic cholestasis of pregnancy (ICP). Methods Human placenta, including normal pregnancies (n = 10) and patients with ICP (n = 10), were collected at term and subject to TNF-alpha measurements. Bile acid-induced TNF-alpha expression and cell apoptosis were evaluated in cultured syncytiotrophoblasts in vitro. Results ICP placental trophoblasts displayed apoptotic histological abnormalities. TNF-alpha levels in ICP tissue were significantly greater than those of controls as measured by quantitative polymerase chain reaction and western blot. Levels of placental TNF-alpha mRNA were positively correlated with serum bile acid concentration in ICP patients. In vitro, lithocholic acid (LCA) significantly enhanced TNF-alpha mRNA at both doses, by 2.07-fold at 15 mu m and by 3.41-fold at 30 mu m, whereas deoxycholic acid mildly increased TNF-alpha mRNA by 1.41-fold at 100 mu m only. LCA treatment produced significantly higher percentage of caspase-3 positive cells than vehicle treatment, rescuable by the addition of a TNF-alpha inhibitor, indicative of apoptosis induced by LCA-TNF-alpha pathway. Conclusion This study shows that the increase of TNF-alpha expression in placental trophoblasts is strongly associated with ICP pathology and is inducible by LCA in vitro, suggesting its potential value in the clinical prevention, diagnosis and treatment of ICP.
学科主题	Gastroenterology & Hepatology
类目[WOS]	Gastroenterology & Hepatology
关键词[WOS]	FETAL-RAT LIVER ; TNF-ALPHA ; BILE-ACIDS ; OXIDATIVE STRESS ; EXPRESSION ; DISEASE ; ROLES ; WOMEN ; BETA
收录类别	SCI
语种	英语
WOS记录号	WOS:000334366900006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/192]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Du, QL,Zhang, YH,Pan, YD,et al. Lithocholic acid-induced placental tumor necrosis factor-alpha upregulation and syncytiotrophoblast cell apoptosis in intrahepatic cholestasis of pregnancy[J]. HEPATOLOGY RESEARCH,2014,44(5):532-541.
APA	Du, QL,Zhang, YH,Pan, YD,&Duan, T.(2014).Lithocholic acid-induced placental tumor necrosis factor-alpha upregulation and syncytiotrophoblast cell apoptosis in intrahepatic cholestasis of pregnancy.HEPATOLOGY RESEARCH,44(5),532-541.
MLA	Du, QL,et al."Lithocholic acid-induced placental tumor necrosis factor-alpha upregulation and syncytiotrophoblast cell apoptosis in intrahepatic cholestasis of pregnancy".HEPATOLOGY RESEARCH 44.5(2014):532-541.