Differential IL-10 production by DCs determines the distinct adjuvant effects of LPS and PTX in EAE induction

	Differential IL-10 production by DCs determines the distinct adjuvant effects of LPS and PTX in EAE induction
	Zhou, HY; Wang, YM; Lian, QS; Yang, B; Ma, YL; Wu, XD; Sun, SH; Liu, YJ; Sun, B
刊名	EUROPEAN JOURNAL OF IMMUNOLOGY
	2014
卷号	44 期号:5 页码:1352-1362
关键词	Dendritic cells EAE IL-10 LPS Pertussis toxin (PTX)
通讯作者	Sun, B (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,Yong-Jun.Liu@BaylorHealth.edu ; bsun@sibs.ac.cn
英文摘要	Pertussis toxin (PTX) is used as an adjuvant to induce EAE in mice as a model of MS. Although LPS and PTX are similar in their ability to mature dendritic cells (DCs) and in their adjuvant properties, here we demonstrate that LPS does not induce EAE development. We also demonstrate that DCs treated with PTX (PTX-DCs) are able to induce EAE, whereas DCs treated with LPS (LPS-DCs) fail to induce EAE. We determine that a key difference between LPS-DCs and PTX-DCs is that LPS-DCs produce larger amounts of IL-10. IL-10(-/-)-DCs treated with LPS promote stronger IFN- and IL-17 production and T-cell proliferation than WT DCs treated with LPS in a coculture system. Finally, we demonstrate that EAE can be successfully induced when IL-10(-/-)-DCs treated with LPS are used as an adjuvant, whereas the use of PTX-DCs overexpressing IL-10 as an adjuvant markedly controls EAE development. These results indicate that the inability of LPS to induce EAE is based on the induction of high levels of IL-10 in the targeted DCs, providing insight into the mechanisms responsible for the induction of EAE.
学科主题	Immunology
类目[WOS]	Immunology
关键词[WOS]	EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS ; HUMAN DENDRITIC CELLS ; REGULATORY T-CELLS ; CYTOKINE GM-CSF ; PERTUSSIS TOXIN ; MULTIPLE-SCLEROSIS ; CLONAL EXPANSION ; TYPE-1 RESPONSES ; HELPER TYPE-1 ; T(H)17 CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000335003200012
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/191]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhou, HY,Wang, YM,Lian, QS,et al. Differential IL-10 production by DCs determines the distinct adjuvant effects of LPS and PTX in EAE induction[J]. EUROPEAN JOURNAL OF IMMUNOLOGY,2014,44(5):1352-1362.
APA	Zhou, HY.,Wang, YM.,Lian, QS.,Yang, B.,Ma, YL.,...&Sun, B.(2014).Differential IL-10 production by DCs determines the distinct adjuvant effects of LPS and PTX in EAE induction.EUROPEAN JOURNAL OF IMMUNOLOGY,44(5),1352-1362.
MLA	Zhou, HY,et al."Differential IL-10 production by DCs determines the distinct adjuvant effects of LPS and PTX in EAE induction".EUROPEAN JOURNAL OF IMMUNOLOGY 44.5(2014):1352-1362.