Autoinhibitory Structure of the WW Domain of HYPB/SETD2 Regulates Its Interaction with the Proline-Rich Region of Huntingtin | |
Gao, YG; Yang, H; Zhao, J; Jiang, YJ; Hu, HY | |
刊名 | STRUCTURE |
2014 | |
卷号 | 22期号:3页码:378-386 |
通讯作者 | Gao, YG (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,yggao@sibcb.ac.cn |
英文摘要 | Huntington's disease (HD) is an autosomally dominant neurodegenerative disorder caused by expansion of polyglutamine (polyQ) in the huntingtin (Htt) protein. Htt yeast two-hybrid protein B (HYPB/SETD2), a histone methyltransferase, directly interacts with Htt and is involved in HD pathology. Using NMR techniques, we characterized a polyproline (polyP) stretch at the C terminus of HYPB, which directly interacts with the following WW domain and leads this domain predominantly to be in a closed conformational state. The solution structure shows that the polyP stretch extends from the back and binds to the WW core domain in a typical binding mode. This autoinhibitory structure regulates interaction between the WW domain of HYPB and the proline-rich region (PRR) of Htt, as evidenced by NMR and immunofluorescence techniques. This work provides structural and mechanistic insights into the intramolecular regulation of the WW domain in Htt-interacting partners and will be helpful for understanding the pathology of HD. |
学科主题 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
关键词[WOS] | HISTONE H3 ; DISEASE PATHOGENESIS ; PROTEIN ; BINDING ; METHYLTRANSFERASE ; IDENTIFICATION ; AGGREGATION ; METHYLATION ; MECHANISMS ; EXPRESSION |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000333025700004 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/159] |
专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
推荐引用方式 GB/T 7714 | Gao, YG,Yang, H,Zhao, J,et al. Autoinhibitory Structure of the WW Domain of HYPB/SETD2 Regulates Its Interaction with the Proline-Rich Region of Huntingtin[J]. STRUCTURE,2014,22(3):378-386. |
APA | Gao, YG,Yang, H,Zhao, J,Jiang, YJ,&Hu, HY.(2014).Autoinhibitory Structure of the WW Domain of HYPB/SETD2 Regulates Its Interaction with the Proline-Rich Region of Huntingtin.STRUCTURE,22(3),378-386. |
MLA | Gao, YG,et al."Autoinhibitory Structure of the WW Domain of HYPB/SETD2 Regulates Its Interaction with the Proline-Rich Region of Huntingtin".STRUCTURE 22.3(2014):378-386. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论