BS69/ZMYND11 Reads and Connects Histone H3.3 Lysine 36 Trimethylation-Decorated Chromatin to Regulated Pre-mRNA Processing

	BS69/ZMYND11 Reads and Connects Histone H3.3 Lysine 36 Trimethylation-Decorated Chromatin to Regulated Pre-mRNA Processing
	Guo, R; Zheng, LJ; Park, JW; Lv, RT; Chen, H; Jiao, FF; Xu, WQ; Mu, SR; Wen, H; Qiu, JS
刊名	MOLECULAR CELL
	2014
卷号	56 期号:2 页码:298-310
通讯作者	Xing, Y (reprint author), Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, CHS 33-228,650 Charles E Young Dr South, Los Angeles, CA 90095 USA.,yxing@ucla.edu ; fei_lan@fudan.edu.cn ; yshi@hms.harvard.edu
英文摘要	BS69 (also called ZMYND11) contains tandemly arranged PHD, BROMO, and PWWP domains, which are chromatin recognition modalities. Here, we show that BS69 selectively recognizes histone variant H3.3 lysine 36 trimethylation (H3.3K36me3) via its chromatin-binding domains. We further identify BS69 association with RNA splicing regulators, including the U5 snRNP components of the spliceosome, such as EFTUD2. Remarkably, RNA sequencing shows that BS69 mainly regulates intron retention (IR), which is the least understood RNA alternative splicing event in mammalian cells. Biochemical and genetic experiments demonstrate that BS69 promotes IR by antagonizing EFTUD2 through physical interactions. We further show that regulation of IR by BS69 also depends on its binding to H3K36me3-decorated chromatin. Taken together, our study identifies an H3.3K36me3-specific reader and a regulator of IR and reveals that BS69 connects histone H3.3K36me3 to regulated RNA splicing, providing significant, important insights into chromatin regulation of pre-mRNA processing.
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	INTRON RETENTION ; TRANSCRIPTION ELONGATION ; SACCHAROMYCES-CEREVISIAE ; PEDIATRIC GLIOBLASTOMA ; RIBOSOMAL TRANSLOCASE ; DRIVER MUTATIONS ; BINDING MODULES ; FISSION YEAST ; VARIANT H3.3 ; PWWP DOMAIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000344484600011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/139]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Guo, R,Zheng, LJ,Park, JW,et al. BS69/ZMYND11 Reads and Connects Histone H3.3 Lysine 36 Trimethylation-Decorated Chromatin to Regulated Pre-mRNA Processing[J]. MOLECULAR CELL,2014,56(2):298-310.
APA	Guo, R.,Zheng, LJ.,Park, JW.,Lv, RT.,Chen, H.,...&Shi, Y.(2014).BS69/ZMYND11 Reads and Connects Histone H3.3 Lysine 36 Trimethylation-Decorated Chromatin to Regulated Pre-mRNA Processing.MOLECULAR CELL,56(2),298-310.
MLA	Guo, R,et al."BS69/ZMYND11 Reads and Connects Histone H3.3 Lysine 36 Trimethylation-Decorated Chromatin to Regulated Pre-mRNA Processing".MOLECULAR CELL 56.2(2014):298-310.