Identification and characterization of epithelial cells derived from human ovarian follicular fluid

	Identification and characterization of epithelial cells derived from human ovarian follicular fluid
	Lai, DM; Xu, MH; Zhang, QW; Chen, YF; Li, T; Wang, Q; Gao, YM; Wei, CS
刊名	Stem Cell Research & Therapy
	2015
卷号	6 期号:1 页码:13-13
通讯作者	Lai, DM (reprint author), Shanghai Jiao Tong Univ, Sch Med, Int Peace Matern & Child Hlth Hosp, Ctr Res Lab, Shanghai 200030, Peoples R China.,laidongmei@hotmail.com ; weics2003@hotmail.com
英文摘要	Introduction: Follicular fluid is important for follicular development and oocyte maturation. Evidence suggests that follicular fluid is not only rich in proteins but cells. Besides oocytes, the follicular fluid contains granulosa, thecal, and ovarian surface epithelial cells, and both granulosa and thecal cells are well-characterized. However, epithelial cells in follicular fluid are poorly studied. This study aims to isolate and characterize in vitro epithelial cells that originate from human ovarian follicular fluid retrieved in the assisted fertilization program. Methods: Follicular fluid samples were collected from 20 women in the assisted reproduction program. Epithelial cells were characterized by flow cytometry assay, immunofluorescence staining, real-time PCR, and time lapse photography. Results: Epithelial cell cultures were established from 18 samples. A small population of epithelial cells expresses germ-line stem cell markers, such as octamer-binding transcription factor 4 (OCT4), NANOG, and DEAD box polypeptide 4 (DDX4). In the epithelial cell culture system, oocyte-like cells formed spontaneously in vitro and expressed the following transcription markers: deleted in azoospermia-like (DAZL), developmental pluripotency associated protein 3 stella-related protein (STELLA), zona pellucida gene family C (ZPC), Syntaptonemal complex protein (SCP), and growth and differentiation factor 9 (GDF9). Some of the oocyte-like cells developed a zona pellucida-like structure. Both the symmetric and asymmetric division split of epithelial cells and early developing oocytes were observed using time lapse photography. Cell colonies were formed during epithelial culturing, which maintained and proliferated in an undifferentiated way on the feeder layer and expressed some pluripotency markers. These colonies differentiated in vitro into various somatic cell types in all three germ layers, but did not form teratoma when injected into immunodeficient mice. Furthermore, these epithelial cells could be differentiated directly to functional hepatocyte-like cells, which do not exist in ovarian tissues. Conclusions: The epithelial cells derived from follicular fluid are a potential stem cell source with a pluripotent/multipotent character for safe application in oogenesis and regenerative medicine.
学科主题	Cell Biology; Research & Experimental Medicine
类目[WOS]	Cell Biology ; Medicine, Research & Experimental
关键词[WOS]	EMBRYONIC STEM-CELLS ; ACTIVE GERM-CELLS ; SURFACE EPITHELIUM ; MAMMALIAN OVARY ; GRANULOSA-CELLS ; IN-VITRO ; FOLLICLES ; DIFFERENTIATION ; RENEWAL ; FOLLICULOGENESIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000353279300001
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/104]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Lai, DM,Xu, MH,Zhang, QW,et al. Identification and characterization of epithelial cells derived from human ovarian follicular fluid[J]. Stem Cell Research & Therapy,2015,6(1):13-13.
APA	Lai, DM.,Xu, MH.,Zhang, QW.,Chen, YF.,Li, T.,...&Wei, CS.(2015).Identification and characterization of epithelial cells derived from human ovarian follicular fluid.Stem Cell Research & Therapy,6(1),13-13.
MLA	Lai, DM,et al."Identification and characterization of epithelial cells derived from human ovarian follicular fluid".Stem Cell Research & Therapy 6.1(2015):13-13.