Combination of inflammation-related cytokines promotes long-term muscle stem cell expansion

	Combination of inflammation-related cytokines promotes long-term muscle stem cell expansion
	Fu, X; Xiao, J; Wei, YN; Li, S; Liu, Y; Yin, J; Sun, K; Sun, H; Wang, HT; Zhang, ZK
刊名	CELL RESEARCH
	2015
卷号	25 期号:6 页码:655-673
关键词	muscle stem cells T cells muscle regeneration inflammation long-term expansion stemness maintenance
通讯作者	Hu, P (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,hongyanwang@sibcb.ac.cn ; hup@sibcb.ac.cn
英文摘要	Muscle stem cells (MuSCs, satellite cells) are the major contributor to muscle regeneration. Like most adult stem cells, long-term expansion of MuSCs in vitro is difficult. The in vivo muscle regeneration abilities of MuSCs are quickly lost after culturing in vitro, which prevents the potential applications of MuSCs in cell-based therapies. Here, we establish a system to serially expand MuSCs in vitro for over 20 passages by mimicking the endogenous microenvironment. We identified that the combination of four pro-inflammatory cytokines, IL-1 alpha, IL-13, TNF-alpha, and IFN-gamma, secreted by T cells was able to stimulate MuSC proliferation in vivo upon injury and promote serial expansion of MuSCs in vitro. The expanded MuSCs can replenish the endogenous stem cell pool and are capable of repairing multiple rounds of muscle injuries in vivo after a single transplantation. The establishment of the in vitro system provides us a powerful method to expand functional MuSCs to repair muscle injuries.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	REGULATORY T-CELLS ; SKELETAL-MUSCLE ; SATELLITE CELL ; SELF-RENEWAL ; MAINTAIN QUIESCENCE ; MUSCULAR-DYSTROPHY ; SECRETORY ORGAN ; GENE-EXPRESSION ; REGENERATION ; ACTIVATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000355554800005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/72]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Fu, X,Xiao, J,Wei, YN,et al. Combination of inflammation-related cytokines promotes long-term muscle stem cell expansion[J]. CELL RESEARCH,2015,25(6):655-673.
APA	Fu, X.,Xiao, J.,Wei, YN.,Li, S.,Liu, Y.,...&Hu, P.(2015).Combination of inflammation-related cytokines promotes long-term muscle stem cell expansion.CELL RESEARCH,25(6),655-673.
MLA	Fu, X,et al."Combination of inflammation-related cytokines promotes long-term muscle stem cell expansion".CELL RESEARCH 25.6(2015):655-673.