An epididymis-specific carboxyl esterase CES5A is required for sperm capacitation and male fertility in the rat

	An epididymis-specific carboxyl esterase CES5A is required for sperm capacitation and male fertility in the rat
	Ru, YF; Xue, HM; Ni, ZM; Xia, D; Zhou, YC; Zhang, YL
刊名	ASIAN JOURNAL OF ANDROLOGY
	2015
卷号	17 期号:2 页码:292-297
关键词	carboxylesterase CES5A epididymis male fertility sperm capacitation
通讯作者	Zhou, YC (reprint author), Chinese Acad Sci, Shanghai Key Lab Mol Androl, State Key Lab Mol Biol, Inst Biochem & Cell Biol,Shanghai Inst Biol Sci, Shanghai, Peoples R China.,zhouych@sibcb.ac.cn ; ylzhang@sibcb.ac.cn
英文摘要	Despite the fact that the phenomenon of capacitation was discovered over half century ago and much progress has been made in identifying sperm events involved in capacitation, few specific molecules of epididymal origin have been identified as being directly involved in this process in vivo . Previously, our group cloned and characterized a carboxyl esterase gene Ces5a in the rat epididymis. The CES5A protein is mainly expressed in the corpus and cauda epididymidis and secreted into the corresponding lumens. Here, we report the function of CES5A in sperm maturation. By local injection of Lentivirus -mediated siRNA in the CES5A -expressing region of the rat epididymis, Ces5a -knockdown animal models were created. These animals exhibited an inhibited sperm capacitation and a reduction in male fertility. These results suggest that CES5A plays an important role in sperm maturation and male fertility.
学科主题	Endocrinology & Metabolism; Urology & Nephrology
类目[WOS]	Andrology ; Urology & Nephrology
关键词[WOS]	PROTEIN-TYROSINE PHOSPHORYLATION ; DROSOPHILA-MELANOGASTER ; REPRODUCTIVE-SYSTEM ; MOUSE SPERMATOZOA ; MATURATION ; GENE ; CHOLESTEROL ; OVEREXPRESSION ; IDENTIFICATION ; LOCALIZATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000352069700025
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/18]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ru, YF,Xue, HM,Ni, ZM,et al. An epididymis-specific carboxyl esterase CES5A is required for sperm capacitation and male fertility in the rat[J]. ASIAN JOURNAL OF ANDROLOGY,2015,17(2):292-297.
APA	Ru, YF,Xue, HM,Ni, ZM,Xia, D,Zhou, YC,&Zhang, YL.(2015).An epididymis-specific carboxyl esterase CES5A is required for sperm capacitation and male fertility in the rat.ASIAN JOURNAL OF ANDROLOGY,17(2),292-297.
MLA	Ru, YF,et al."An epididymis-specific carboxyl esterase CES5A is required for sperm capacitation and male fertility in the rat".ASIAN JOURNAL OF ANDROLOGY 17.2(2015):292-297.