Identification of a novel nanoRNase in Bartonella

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	Identification of a novel nanoRNase in Bartonella
	Liu, Ma Feng 1,2; Cescau, Sandra 3; Mechold, Undine 3; Wang, Jing4 ; Cohen, Dorit 5; Danchin, Antoine 6; Boulouis, Henri-Jean 1; Biville, Francis 1,3
刊名	MICROBIOLOGY-SGM
	2012-04-01
卷号	158 页码:886-895
ISSN号	1350-0872
通讯作者	Biville, F (reprint author), UMR BIPAR INRA AFSSA ENVA, 23 Ave Gen Gaulle, F-94700 Maisons Alfort, France.
产权排序	4
英文摘要	In Escherichia coli, only one essential oligoribonuclease (Orn) can degrade oligoribonucleotides of five residues and shorter in length (nanoRNA). In Bacillus subtilis, NrnA and NrnB, which do not show any sequence similarity to Orn, have been identified as functional analogues of Orn. Sequence comparisons did not identify orn, nrnA or nrnB homologues in the genomes of the Chlamydia/Cyanobacteria and Alphaproteobacteria family members. Screening a genomic library from Bartonella birtlesii, a member of the Alphaproteobacteria, for genes that can complement a conditional orn mutant. in E. coli, we identified BA0969 (NrnC) as a functional analogue of Orn. NrnC is highly conserved (more than 80% identity) in the Bartonella genomes sequenced to date. Biochemical characterization showed that this protein exhibits oligo RNA degradation activity (nanoRNase activity). Like Orn from E. coli, NrnC is inhibited by micromolar amounts of 3'-phosphoadenosine 5'-phosphate in vitro. NrnC homologues are widely present in genomes of Alphaproteobacteria. Knock down of nrnC decreases the growth ability of Bartonella henselae, demonstrating the importance of nanoRNase activity in this bacterium.
学科主题	Physiological psychology/Biological Psychology
收录类别	SCI
项目简介	We thank Dr D. Gillaspie and Dr B. E. Anderson (University of South Florida) for the generous gift of plasmids pNS2Amp and pNS2Trc. This work was supported by the Pasteur Institute, the Centre National de la Recherche Scientifique (URA2172), the Agence Nationale pour la Recherche (ANR-06-MIME-019-01) and TARPOL (grant KBBE-212894). M. F. L. was supported by the China Scholarship Council.
原文出处	http://mic.sgmjournals.org/content/158/Pt_4/886.full.pdf
语种	英语
WOS记录号	WOS:000303633200003
内容类型	期刊论文
源URL	[http://ir.psych.ac.cn/handle/311026/12848]
专题	心理研究所_中国科学院心理健康重点实验室
作者单位	1.UMR BIPAR INRA AFSSA ENVA, F-94700 Maisons Alfort, France 2.Jilin Univ, Inst Zoonosis, Minist Educ, Key Lab Zoonosis, Changchun 130062, Peoples R China 3.Inst Pasteur, F-75015 Paris, France 4.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China 5.Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel 6.AMAbiotics SAS, F-91000 Evry, France
推荐引用方式 GB/T 7714	Liu, Ma Feng,Cescau, Sandra,Mechold, Undine,et al. Identification of a novel nanoRNase in Bartonella[J]. MICROBIOLOGY-SGM,2012,158:886-895.
APA	Liu, Ma Feng.,Cescau, Sandra.,Mechold, Undine.,Wang, Jing.,Cohen, Dorit.,...&Biville, Francis.(2012).Identification of a novel nanoRNase in Bartonella.MICROBIOLOGY-SGM,158,886-895.
MLA	Liu, Ma Feng,et al."Identification of a novel nanoRNase in Bartonella".MICROBIOLOGY-SGM 158(2012):886-895.