Structure change and intermediate's lifetime of bacteriorhodopsin and its multipoint mutants

CORC > 西安光学精密机械研究所 > 中国科学院西安光学精密机械研究所 > 瞬态光学技术国家重点实验室

	Structure change and intermediate's lifetime of bacteriorhodopsin and its multipoint mutants
	Jin Wei-Hua 1; Cao Jun-Wei 1; Wang You-Liang 1; Xiong Gui-Guang 2; Yao Bao-Li 3; Lei Ming3
刊名	chemical journal of chinese universities-chinese
	2007-12-10
卷号	28 期号:12 页码:2321-2326
关键词	bacterorhodopisin (BR) mutant Resonance Raman spectrum PVA
英文摘要	the site-specific residue replacements e194q, i119t/t121s/a126t and i119t/t121s/a126t/ e194q were introduced into bop gene and expressed in halobacterium salinarium l-33 with the vector pxl-novr, respectively. the photoreaction of three mutants bre194q bri119t/t121s/a126t and bri119t/t121s/a126t/e194q (the quadruple mutant) of bacteriorhodopsin (br) and wt-br (wild type br) was investigated in solution and in pva films at ph=7.0 by uv-vis absorption spectrum and resonance raman spectra. the lifetime of m intermediate of the three mutants in pva films were recorded by employing photomicrography. in distilled water, the visible absorption maximum of bri119t/t121s/a126t and bri119t/t121s/a126t/e194q mutants were blue shifted notably by 11 and 12 nm respectively and the bre194q's was weakly red shifted in comparison with wt-br. in pva film, bri119t/t121s/a126t/e194q mutant caused a blue shift of the visible absorption spectrum from 568 to 557 nm. the characteristic bands of resonance raman spectra of bri119t/t121s/a126t/e194q mutant were observed at 1334cm(-1) of the l intermediate, 1200, 1328, 1530 and 1549 cm(-1) of the n intermediate and 1567 and 1573 cm(-1) of m intermediate in solution. however, the 1334 and 1549 cm(-1) bands of the quadruple mutant disappeared and the intensity of 1187 cm(-1) band decreased in pva film. the lifetime of m intermediate of bre194q was less than 1.0 s by photomicrography. the lifetime of m intermediates of bri119t/t121s/a126t and bri119t/t121s/a126t/e194q were 3.0 s and 2.0 s, respectively.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	halobacterium-halobium ; deprotonation ; photocycle ; devices
收录类别	SCI
语种	中文
WOS记录号	WOS:000251953400021
公开日期	2015-08-25
内容类型	期刊论文
源URL	[http://ir.opt.ac.cn/handle/181661/25192]
专题	西安光学精密机械研究所_瞬态光学技术国家重点实验室
作者单位	1.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China 2.Wuhan Univ, Sch Phys & Technol, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transiet Opt Technol, Xian 710068, Peoples R China
推荐引用方式 GB/T 7714	Jin Wei-Hua,Cao Jun-Wei,Wang You-Liang,et al. Structure change and intermediate's lifetime of bacteriorhodopsin and its multipoint mutants[J]. chemical journal of chinese universities-chinese,2007,28(12):2321-2326.
APA	Jin Wei-Hua,Cao Jun-Wei,Wang You-Liang,Xiong Gui-Guang,Yao Bao-Li,&Lei Ming.(2007).Structure change and intermediate's lifetime of bacteriorhodopsin and its multipoint mutants.chemical journal of chinese universities-chinese,28(12),2321-2326.
MLA	Jin Wei-Hua,et al."Structure change and intermediate's lifetime of bacteriorhodopsin and its multipoint mutants".chemical journal of chinese universities-chinese 28.12(2007):2321-2326.