Dihydroisotanshinone I protects against menadione-induced toxicity in a primary culture of rat hepatocytes

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Dihydroisotanshinone I protects against menadione-induced toxicity in a primary culture of rat hepatocytes
	Ip, SP ; Yang, H ; Sun, HD; Che, CT
刊名	PLANTA MEDICA
	2002-12-01
卷号	68 期号:12 页码:1077-1081
关键词	Salvia trijuga Lamiaceae dihydroisotanshinone I menadione liver damage free radical scavenger lipid peroxidation
英文摘要	Dihydroisotanshinone I is a phenanthrenequinone derivative isolated from the roots of Salvia trijuga Diels. The present study demonstrated the hepatoprotective effect of dihydroisotanshinone I against menadione-induced cytotoxicity in a primary culture of rat hepatocytes. Pretreating the cells with dihydroisotanshinone I at concentrations ranging from 2.5 muM to 20 muM for 24 hours caused dose-dependent protection against hepatotoxicity induced by menadione. Intracellular glutathione level and activity of DT-diaphorase have been suggested to play important roles in menadione-induced cytotoxicity. However, treating the hepatocytes with 20 muM dihydroisotanshinone I for 24 hours did not cause a significant change in glutathione level and DT-diaphorase activity. On the contrary, adding dihydroisotanshinone I to freshly isolated hepatocytes at concentrations between 50 nM to 200 nM inhibited NADH-induced superoxide production dose-dependently as indicated by the decrease of lucigenin-amplified chemiluminescence. In addition, dihydroisotanshinone I at concentrations ranging from 5 muM to 20 muM inhibited tert-butyl hydroperoxide-induced lipid peroxidation dose-dependently in isolated hepatocytes as indicated by the level of malondialdehyde. These results suggest that the protective action of dihydroisotanshinone I against menadione-induced hepatotoxicity is attributed to its antioxidant properties including the free radical scavenging activity and inhibition of lipid peroxidation.
类目[WOS]	Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]	Plant Sciences ; Pharmacology & Pharmacy
关键词[WOS]	INDUCED OXIDATIVE STRESS ; DT-DIAPHORASE ; SCHISANDRIN-B ; CELLS ; HEPATOTOXICITY ; GLUTATHIONE
收录类别	SCI
语种	英语
WOS记录号	WOS:000180439100006
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/23973]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Univ Hong Kong, Sch Chinese Med, Shatin, Hong Kong, Peoples R China 2.Yunnan Univ, Dept Appl Chem, Kunming 650091, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Lab Phytochem, Kunming, Peoples R China
推荐引用方式 GB/T 7714	Ip, SP,Yang, H,Sun, HD,et al. Dihydroisotanshinone I protects against menadione-induced toxicity in a primary culture of rat hepatocytes[J]. PLANTA MEDICA,2002,68(12):1077-1081.
APA	Ip, SP,Yang, H,Sun, HD,&Che, CT.(2002).Dihydroisotanshinone I protects against menadione-induced toxicity in a primary culture of rat hepatocytes.PLANTA MEDICA,68(12),1077-1081.
MLA	Ip, SP,et al."Dihydroisotanshinone I protects against menadione-induced toxicity in a primary culture of rat hepatocytes".PLANTA MEDICA 68.12(2002):1077-1081.