Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design
	Qin, Wei; Ding, Jiawang
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2014-08
卷号	199 页码:284-290
关键词	Nanoporous gold Carbon nanotubes Amperometry Acetylcholinesterase Pesticides
ISSN号	0925-4005
通讯作者	Qin, W (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, YICCAS, Yantai Inst Coastal Zone Res YIC,Key Lab Coastal, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn ; dan.du@mail.ccnu.edu.cn
产权排序	[Ding, Jiawang; Zhang, Hongxia; Qin, Wei] Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, YICCAS, Yantai Inst Coastal Zone Res YIC,Key Lab Coastal, Yantai 264003, Shandong, Peoples R China; [Jia, Falong; Du, Dan] Huazhong Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China
中文摘要	An electrochemical sensing platform based on assembly of carbon nanotubes on a nanoporous gold electrode is described for highly sensitive detection of organophosphate pesticides. The nanoporous gold film (NPG) electrode is fabricated by an alloying/dealloying process, which possess high electroactive surface area and is an excellent substrate for sensor design. The NPG functionalized with cysteamine allows the immobilization of carbon nanotubes on the electrode with the self-assembly technique. The carboxylated carbon nanotubes are further linkered with acetylcholinesterase (AChE) for amperometric sensing of pesticides. The immobilized AChE, as a model, shows excellent activity to its substrate and allows a quantitative measurement of organophosphate pesticides. Under the optimal experimental conditions, the inhibition of malathion is proportional to its concentration in the range of 0.001-0.5 mu g mL(-1) with a detection limit of 0.5 ng mL(-1). The proposed method shows good reproducibility and high stability, which provides a new avenue for electrochemical biosensor design. (C) 2014 Elsevier B.V. All rights reserved.
学科主题	Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation
研究领域[WOS]	National Natural Science Foundation of China [21207156] ; Science and Technology Project of Yantai [2012132] ; Taishan Scholar Program of Shandong Province [TS20081159]
关键词[WOS]	RAT-BRAIN ACETYLCHOLINESTERASE ; LINKED CHITOSAN COMPOSITE ; MODIFIED AU ELECTRODE ; ORGANOPHOSPHATE PESTICIDES ; ELECTROCHEMICAL PROPERTIES ; FILM ELECTRODES ; SURFACE-AREA ; POROUS GOLD ; NANOPARTICLES ; IMMOBILIZATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000336878000040
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/8724]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Qin, Wei
推荐引用方式 GB/T 7714	Qin, Wei,Ding, Jiawang. Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design[J]. SENSORS AND ACTUATORS B-CHEMICAL,2014,199:284-290.
APA	Qin, Wei,&Ding, Jiawang.(2014).Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design.SENSORS AND ACTUATORS B-CHEMICAL,199,284-290.
MLA	Qin, Wei,et al."Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design".SENSORS AND ACTUATORS B-CHEMICAL 199(2014):284-290.