Phosphomonoesterase Activities, Kinetics and Thermodynamics in a Paddy Soil After Receiving Swine Manure for Six Years

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Phosphomonoesterase Activities, Kinetics and Thermodynamics in a Paddy Soil After Receiving Swine Manure for Six Years
	Li Liang 1; Liang Xin-Qiang 1; Li Hua 2; Ji Yuan-Jing 3; Liu Jin 3; Ye Yu-Shi 3; Tian Guang-Ming 1; Chen Ying-Xu 1; Luo Yong-Ming4
刊名	PEDOSPHERE
	2015-04-01
卷号	25 期号:2 页码:294-306
关键词	activation energy activation enthalpy enzyme-catalyzed reaction maximal velocity Michaelis constant
ISSN号	1002-0160
通讯作者	Liang, XQ (reprint author), Zhejiang Univ, Inst Environm Sci & Technol, Coll Environm & Resources Sci, Hangzhou 310058, Zhejiang, Peoples R China. liang410@zju.edu.cn
产权排序	[Li Liang; Liang Xin-Qiang; Tian Guang-Ming; Chen Ying-Xu] Zhejiang Univ, Inst Environm Sci & Technol, Coll Environm & Resources Sci, Hangzhou 310058, Zhejiang, Peoples R China; [Li Hua] Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China; [Ji Yuan-Jing; Liu Jin; Ye Yu-Shi] Key Lab Water Pollut Control & Environm Safety, Hangzhou 310058, Zhejiang, Peoples R China; [Luo Yong-Ming] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
中文摘要	Soil phosphomonoesterase plays a critical role in controlling phosphorus (P) cycling for crop nutrition, especially in P-deficient soils. A 6-year field experiment was conducted to evaluate soil phosphomonoesterase activities, kinetics and thermodynamics during rice growth stages after consistent swine manure application, to understand the impacts of swine manure amendment rates on soil chemical and enzymatic properties, and to investigate the correlations between soil enzymatic and chemical variables. The experiment was set out in a randomized complete block design with three replicates and five treatments including three swine manure rates (26, 39, and 52 kg P ha(-1), representing low, middle, and high application rates, respectively) and two controls (no-fertilizer and superphosphate at 26 kg P ha(-1)). The results indicated that the grain yield and soil chemical properties were significantly improved with the application of P-based swine manure from 0 to 39 kg P ha(-1); however, the differences between the 39 (M-39) and 52 kg P ha(-1) treatments (M-52) were not significant. The enzymatic property analysis indicated that acid phosphomonoesterase was the predominant phosphomonoesterase in the tested soil. The M-39 and M-52 treatments had relatively high initial velocity (V-0), maximal velocity (V-max), and activation grade (lgN(a)) but low Michaelis constant (K-m), temperature coefficient (Q(10)), activation energy (E-a), and activation enthalpy (Delta H), implying that the M-39 and M-52 treatments could stimulate the enzyme-catalyzed reactions more easily than all other treatments. The correlation analysis showed that the distribution of soil phosphomonoesterase activities mainly followed the distributions of total C and total N. Based on these results, 39 kg P ha(-1) could be recommended as the most appropriate rate of swine manure amendment.
英文摘要	Soil phosphomonoesterase plays a critical role in controlling phosphorus (P) cycling for crop nutrition, especially in P-deficient soils. A 6-year field experiment was conducted to evaluate soil phosphomonoesterase activities, kinetics and thermodynamics during rice growth stages after consistent swine manure application, to understand the impacts of swine manure amendment rates on soil chemical and enzymatic properties, and to investigate the correlations between soil enzymatic and chemical variables. The experiment was set out in a randomized complete block design with three replicates and five treatments including three swine manure rates (26, 39, and 52 kg P ha(-1), representing low, middle, and high application rates, respectively) and two controls (no-fertilizer and superphosphate at 26 kg P ha(-1)). The results indicated that the grain yield and soil chemical properties were significantly improved with the application of P-based swine manure from 0 to 39 kg P ha(-1); however, the differences between the 39 (M-39) and 52 kg P ha(-1) treatments (M-52) were not significant. The enzymatic property analysis indicated that acid phosphomonoesterase was the predominant phosphomonoesterase in the tested soil. The M-39 and M-52 treatments had relatively high initial velocity (V-0), maximal velocity (V-max), and activation grade (lgN(a)) but low Michaelis constant (K-m), temperature coefficient (Q(10)), activation energy (E-a), and activation enthalpy (Delta H), implying that the M-39 and M-52 treatments could stimulate the enzyme-catalyzed reactions more easily than all other treatments. The correlation analysis showed that the distribution of soil phosphomonoesterase activities mainly followed the distributions of total C and total N. Based on these results, 39 kg P ha(-1) could be recommended as the most appropriate rate of swine manure amendment.
学科主题	Soil Science
研究领域[WOS]	Agriculture
关键词[WOS]	SEWAGE-SLUDGE APPLICATION ; LONG-TERM APPLICATION ; PHOSPHATASE-ACTIVITIES ; ENZYME-ACTIVITIES ; MICROBIAL BIOMASS ; MINERAL COMPLEXES ; P FRACTIONS ; OAK LITTER ; SANDY SOIL ; PHOSPHORUS
收录类别	SCI
语种	英语
WOS记录号	WOS:000352117700014
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/8635]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Zhejiang Univ, Inst Environm Sci & Technol, Coll Environm & Resources Sci, Hangzhou 310058, Zhejiang, Peoples R China 2.Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China 3.Key Lab Water Pollut Control & Environm Safety, Hangzhou 310058, Zhejiang, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Li Liang,Liang Xin-Qiang,Li Hua,et al. Phosphomonoesterase Activities, Kinetics and Thermodynamics in a Paddy Soil After Receiving Swine Manure for Six Years[J]. PEDOSPHERE,2015,25(2):294-306.
APA	Li Liang.,Liang Xin-Qiang.,Li Hua.,Ji Yuan-Jing.,Liu Jin.,...&Luo Yong-Ming.(2015).Phosphomonoesterase Activities, Kinetics and Thermodynamics in a Paddy Soil After Receiving Swine Manure for Six Years.PEDOSPHERE,25(2),294-306.
MLA	Li Liang,et al."Phosphomonoesterase Activities, Kinetics and Thermodynamics in a Paddy Soil After Receiving Swine Manure for Six Years".PEDOSPHERE 25.2(2015):294-306.