Cloning and Expression of a Cytosolic HSP90 Gene in Chlorella vulgaris

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	Cloning and Expression of a Cytosolic HSP90 Gene in Chlorella vulgaris
	Liu, Zhengyi 1; Zhang, Lei 2; Pu, Yang 2; Liu, Zhaopu 1; Li, Zhiling 3; Zhao, Yushan 3; Qin, Song1
刊名	BIOMED RESEARCH INTERNATIONAL
	2014
页码	487050
关键词	CHLAMYDOMONAS-REINHARDTII ARABIDOPSIS-THALIANA SEQUENCE ALIGNMENT STRESS TOLERANCE HEAT-STRESS FAMILY SHOCK CHLOROPLAST EVOLUTIONARY PROTEINS
ISSN号	2314-6133
通讯作者	Qin, S (reprint author), Nanjing Agr Univ, Key Lab Marine Biol Jiang Su, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China. sqin@yic.ac.cn
产权排序	[Liu, Zhengyi; Liu, Zhaopu; Qin, Song] Nanjing Agr Univ, Key Lab Marine Biol Jiang Su, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China; [Zhang, Lei; Pu, Yang] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Li, Zhiling; Zhao, Yushan] Shandong Oriental Ocean Sci Tech Co Ltd, Yantai 264003, Peoples R China
中文摘要	Heat shock protein 90 (HSP90), a highly conserved molecular chaperone, plays essential roles in folding, keeping structural integrity, and regulating the subset of cytosolic proteins. We cloned the cDNA of Chlorella vulgaris HSP90 (named CvHSP90) by combining homology cloning with rapid amplification of cDNA ends (RACE). Sequence analysis indicated that CvHSP90 is a cytosolic member of the HSP90 family. Quantitative RT-PCR was applied to determine the expression level of messenger RNA (mRNA) in CvHSP90 under different stress conditions. C. vulgaris was kept in different temperatures (5-45 degrees C) for 1 h. The mRNA expression level of CvHSP90 increased with temperature from 5 to 10 degrees C, went further from 35 to 40 degrees C, and reached the maximum at 40 degrees C. On the other hand, for C. vulgaris kept at 35 degrees C for different durations, the mRNA expression level of CvHSP90 increased gradually and reached the peak at 7 h and then declined progressively. In addition, the expression level of CvHSP90 at 40 or 45 in salinity (parts per thousand) was almost fourfold of that at 25 in salinity (parts per thousand) for 2 h. Therefore, CvHSP90 may be a potential biomarker to monitor environment changes.
英文摘要	Heat shock protein 90 (HSP90), a highly conserved molecular chaperone, plays essential roles in folding, keeping structural integrity, and regulating the subset of cytosolic proteins. We cloned the cDNA of Chlorella vulgaris HSP90 (named CvHSP90) by combining homology cloning with rapid amplification of cDNA ends (RACE). Sequence analysis indicated that CvHSP90 is a cytosolic member of the HSP90 family. Quantitative RT-PCR was applied to determine the expression level of messenger RNA (mRNA) in CvHSP90 under different stress conditions. C. vulgaris was kept in different temperatures (5-45 degrees C) for 1 h. The mRNA expression level of CvHSP90 increased with temperature from 5 to 10 degrees C, went further from 35 to 40 degrees C, and reached the maximum at 40 degrees C. On the other hand, for C. vulgaris kept at 35 degrees C for different durations, the mRNA expression level of CvHSP90 increased gradually and reached the peak at 7 h and then declined progressively. In addition, the expression level of CvHSP90 at 40 or 45 in salinity (parts per thousand) was almost fourfold of that at 25 in salinity (parts per thousand) for 2 h. Therefore, CvHSP90 may be a potential biomarker to monitor environment changes.
学科主题	Biotechnology & Applied Microbiology; Medicine, Research & Experimental
研究领域[WOS]	Biotechnology & Applied Microbiology ; Research & Experimental Medicine
关键词[WOS]	CHLAMYDOMONAS-REINHARDTII ; ARABIDOPSIS-THALIANA ; SEQUENCE ALIGNMENT ; STRESS TOLERANCE ; HEAT-STRESS ; FAMILY ; SHOCK ; CHLOROPLAST ; EVOLUTIONARY ; PROTEINS
收录类别	SCI
语种	英语
WOS记录号	WOS:000333346100001
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/8583]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Nanjing Agr Univ, Key Lab Marine Biol Jiang Su, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Shandong Oriental Ocean Sci Tech Co Ltd, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zhengyi,Zhang, Lei,Pu, Yang,et al. Cloning and Expression of a Cytosolic HSP90 Gene in Chlorella vulgaris[J]. BIOMED RESEARCH INTERNATIONAL,2014:487050.
APA	Liu, Zhengyi.,Zhang, Lei.,Pu, Yang.,Liu, Zhaopu.,Li, Zhiling.,...&Qin, Song.(2014).Cloning and Expression of a Cytosolic HSP90 Gene in Chlorella vulgaris.BIOMED RESEARCH INTERNATIONAL,487050.
MLA	Liu, Zhengyi,et al."Cloning and Expression of a Cytosolic HSP90 Gene in Chlorella vulgaris".BIOMED RESEARCH INTERNATIONAL (2014):487050.