Direct production of bioethanol from Jerusalem artichoke inulin by gene-engineering Saccharomyces cerevisiae 6525 with exoinulinase gene

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Direct production of bioethanol from Jerusalem artichoke inulin by gene-engineering Saccharomyces cerevisiae 6525 with exoinulinase gene
	Wang, L.1,2 ; Du, Y. C.1; Meng, X. F.1; Long, X. H.1; Liu, Z. P.1; Shao, H.2,3
刊名	PLANT BIOSYSTEMS
	2014-01-02
卷号	148 期号:1 页码:133-139
关键词	Bioethanol Jerusalem artichoke inulinase Saccharomyces cerevisiae gene engineering
ISSN号	1126-3504
通讯作者	Long, XH (reprint author), Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China. longxiaohua@njau.edu.cn ; shaohongbochu@126.com
产权排序	[Wang, L.; Du, Y. C.; Meng, X. F.; Long, X. H.; Liu, Z. P.] Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China; [Wang, L.; Shao, H.] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China; [Shao, H.] Qingdao Univ Sci & Technol QUST, Inst Life Sci, Qingdao 266042, Peoples R China
中文摘要	Jerusalem artichoke (Helianthus tuberosus L.), an important crop, containing over 50% inulin in its tubers on a dry weight basis is an agricultural and industrial crop with a great potential for production of ethanol and industrial products. Inulin is a good substrate for bioethanol production. Saccharomyces cerevisiae 6525 can produce high concentrations of ethanol, but it cannot synthesize inulinase. In this study, a new integration vector carrying inuA1 gene encoding exoinulinase was constructed and transformed into 18SrDNA site of industrial strain S. cerevisiae 6525. The obtained transformant, BR8, produced 1.1UmL(-1) inulinase activity within 72h and the dry cell weight reached 12.3gL(-1) within 48h. In a small-scale fermentation, BR8 produced 9.5% (v/v) ethanol, with a productivity rate of 0.385g ethanol per gram inulin, while wild-type S. cerevisiae 6525 produced only 3.3% (v/v) ethanol in the same conditions. In a 5-L fermentation, BR8 produced 14.0% (v/v) ethanol in fermentation medium containing inulin and 1% (w/v) (NH4)(2)SO4. The engineered S. cerevisiae 6525 carrying inuA1 converted pure nonhydrolyzed inulin directly into high concentrations of ethanol.
英文摘要	Jerusalem artichoke (Helianthus tuberosus L.), an important crop, containing over 50% inulin in its tubers on a dry weight basis is an agricultural and industrial crop with a great potential for production of ethanol and industrial products. Inulin is a good substrate for bioethanol production. Saccharomyces cerevisiae 6525 can produce high concentrations of ethanol, but it cannot synthesize inulinase. In this study, a new integration vector carrying inuA1 gene encoding exoinulinase was constructed and transformed into 18SrDNA site of industrial strain S. cerevisiae 6525. The obtained transformant, BR8, produced 1.1UmL(-1) inulinase activity within 72h and the dry cell weight reached 12.3gL(-1) within 48h. In a small-scale fermentation, BR8 produced 9.5% (v/v) ethanol, with a productivity rate of 0.385g ethanol per gram inulin, while wild-type S. cerevisiae 6525 produced only 3.3% (v/v) ethanol in the same conditions. In a 5-L fermentation, BR8 produced 14.0% (v/v) ethanol in fermentation medium containing inulin and 1% (w/v) (NH4)(2)SO4. The engineered S. cerevisiae 6525 carrying inuA1 converted pure nonhydrolyzed inulin directly into high concentrations of ethanol.
学科主题	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	ETHANOL-PRODUCTION ; GROWTH ; EXPRESSION ; TOLERANCE ; TUBERS ; CHINA
收录类别	SCI
语种	英语
WOS记录号	WOS:000333880000019
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/8564]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室中国科学院烟台海岸带研究所
作者单位	1.Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China 3.Qingdao Univ Sci & Technol QUST, Inst Life Sci, Qingdao 266042, Peoples R China
推荐引用方式 GB/T 7714	Wang, L.,Du, Y. C.,Meng, X. F.,et al. Direct production of bioethanol from Jerusalem artichoke inulin by gene-engineering Saccharomyces cerevisiae 6525 with exoinulinase gene[J]. PLANT BIOSYSTEMS,2014,148(1):133-139.
APA	Wang, L.,Du, Y. C.,Meng, X. F.,Long, X. H.,Liu, Z. P.,&Shao, H..(2014).Direct production of bioethanol from Jerusalem artichoke inulin by gene-engineering Saccharomyces cerevisiae 6525 with exoinulinase gene.PLANT BIOSYSTEMS,148(1),133-139.
MLA	Wang, L.,et al."Direct production of bioethanol from Jerusalem artichoke inulin by gene-engineering Saccharomyces cerevisiae 6525 with exoinulinase gene".PLANT BIOSYSTEMS 148.1(2014):133-139.